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Link to original content: https://www.ncbi.nlm.nih.gov/pubmed/33996364
Heat-killed Lactobacillus plantarum L-137 attenuates obesity and associated metabolic abnormalities in C57BL/6 J mice on a high-fat diet - PubMed Skip to main page content
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. 2021;40(2):84-91.
doi: 10.12938/bmfh.2020-040. Epub 2020 Nov 14.

Heat-killed Lactobacillus plantarum L-137 attenuates obesity and associated metabolic abnormalities in C57BL/6 J mice on a high-fat diet

Rieko Yoshitake  1 , Yoshitaka Hirose  1 , Shinji Murosaki  2 , Goro Matsuzaki  1
Affiliations

Heat-killed Lactobacillus plantarum L-137 attenuates obesity and associated metabolic abnormalities in C57BL/6 J mice on a high-fat diet

Rieko Yoshitake et al. Biosci Microbiota Food Health. 2021.

Abstract

Heat-killed Lactobacillus plantarum L-137 (HK L-137) has anti-allergic, antitumor, and antiviral effects in mice, as well as an anti-inflammatory effect in rats with metabolic syndrome through regulation of immunity. To evaluate the influence of HK L-137 on chronic inflammation in mice with diet-induced obesity, C57BL/6 J mice were fed a normal diet (16% of energy as fat) or a high-fat diet (62% of energy as fat) with or without 0.002% HK L-137 for 4 to 20 weeks. It was found that HK L-137 supplementation alleviated weight gain and elevation of plasma glucose, cholesterol, alanine aminotransferase, and aspartate transaminase levels in mice with diet-induced obesity. Expression of several inflammation-related genes, including F4/80, CD11c, and IL-1β, in the epididymal adipose tissue of these mice was significantly downregulated by HK L-137. In addition, plasma levels of lipopolysaccharide-binding protein, a marker of endotoxemia, tended to be decreased by administration of HK L-137. These findings suggest that HK L-137 supplementation ameliorates obesity-induced metabolic abnormalities and adipose tissue inflammation, possibly through improvement of intestinal permeability.

Keywords: Lactobacillus; adipose tissue; cholesterol; inflammation; macrophage; obesity.

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Figures

Fig. 1.
Fig. 1.
Effect of HK L-137 on body weight and food intake in C57BL/6 J mice fed the HFD. Mice were fed a normal diet (ND, white bars) or a high-fat diet without (HFD(−), black bar) or with (HFD(+), gray bar) 0.002% HK L-137 for 20 weeks. Body weight changes (A) and total food intake (B) are shown. Values are means, with their SDs represented by vertical bars (n=32 at 0–4 weeks; n=27 at 5–8 weeks; n=22 at 9–12 weeks; n=17 at 13–16 weeks; n=12 at 17–20 weeks). Significant differences between the HFD(−) and HFD(+) groups were evaluated by Student’s t-test. Differences from the HFD(−) group are indicated as follows: *p<0.05; †p<0.1. Data from the ND group are shown for reference.
Fig. 2.
Fig. 2.
Effect of HK L-137 on the expression of pro-inflammatory genes in eWAT. The expression levels of MCP1 (A), F4/80 (B), CD11c (C), TNF-α (D), and IL-1β (E) are shown. Expression of the target genes was normalized for that of the endogenous control (GAPDH). Values are expressed as the mean ± SD (n=5 mice per group). Significant differences between the HFD(−) and HFD(+) groups were evaluated by Student’s t-test. Differences from the HFD(−) group are indicated as follows: *p<0.05; †p<0.1. Data from the ND group are shown for reference (n=5). MCP1: monocyte chemoattractant protein 1; CD11c: cluster of differentiation 11c; TNF-α: tumor necrosis factor-α; IL-1β: interleukin-1β; eWAT: epididymal white adipose tissue; ND: normal diet; HFD(−): high-fat diet without 0.002% HK L-137; HFD(+): high-fat diet with 0.002% HK L-137.
Fig. 3.
Fig. 3.
Effect of HK L-137 on plasma LBP levels in C57BL/6 J mice fed the HFD. Plasma LBP levels are shown. Values are expressed as the mean ± SD (n=5 mice per group). †p<0.1 by Student’s t-test. Data from the ND group are shown for reference (n=5). LBP: lipopolysaccharide-binding protein; ND: normal diet; HFD(−): high-fat diet without 0.002% HK L-137; HFD(+): high-fat diet with 0.002% HK L-137.
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