iBet uBet web content aggregator. Adding the entire web to your favor.
iBet uBet web content aggregator. Adding the entire web to your favor.



Link to original content: https://www.ncbi.nlm.nih.gov/pubmed/27509516
Effects of Long-Term Exposure to 60 GHz Millimeter-Wavelength Radiation on the Genotoxicity and Heat Shock Protein (Hsp) Expression of Cells Derived from Human Eye - PubMed Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016 Aug 8;13(8):802.
doi: 10.3390/ijerph13080802.

Effects of Long-Term Exposure to 60 GHz Millimeter-Wavelength Radiation on the Genotoxicity and Heat Shock Protein (Hsp) Expression of Cells Derived from Human Eye

Shin Koyama  1 , Eijiro Narita  2 , Yoko Shimizu  3 , Yukihisa Suzuki  4 , Takeo Shiina  5 , Masao Taki  6 , Naoki Shinohara  7 , Junji Miyakoshi  8
Affiliations

Effects of Long-Term Exposure to 60 GHz Millimeter-Wavelength Radiation on the Genotoxicity and Heat Shock Protein (Hsp) Expression of Cells Derived from Human Eye

Shin Koyama et al. Int J Environ Res Public Health. .

Abstract

Human corneal epithelial (HCE-T) and human lens epithelial (SRA01/04) cells derived from the human eye were exposed to 60 gigahertz (GHz) millimeter-wavelength radiation for 24 h. There was no statistically significant increase in the micronucleus (MN) frequency in cells exposed to 60 GHz millimeter-wavelength radiation at 1 mW/cm² compared with sham-exposed controls and incubator controls. The MN frequency of cells treated with bleomycin for 1 h provided positive controls. The comet assay, used to detect DNA strand breaks, and heat shock protein (Hsp) expression also showed no statistically significant effects of exposure. These results indicate that exposure to millimeter-wavelength radiation has no effect on genotoxicity in human eye cells.

Keywords: cellular genotoxicity; comet assay; heat shock protein; human eye cells; long-term exposure; micronucleus formation; millimeter-waves.

PubMed Disclaimer

Figures

Figure 1
Figure 1
The built-in incubator of the 60 GHz exposure system (a) and an inside view of the incubator (b). The chamber of the exposure system was maintained under controlled conditions similar to those in an incubator, i.e., an atmosphere of 95% air and 5% CO2 at a relative humidity of >95% and a temperature of 37 °C.
Figure 2
Figure 2
Micronucleus frequency in cells exposed to millimeter-wavelength radiation at 60 GHz for 24 h: HCE-T cells (a) and SRA01/04 cells (b). Treatment with bleomycin (10 µg/mL) provided the positive controls. Data are presented as the mean ± SD from three independent experiments. Asterisks ** indicate p < 0.01. f = 0.1, (1−β) = 0.9903.
Figure 3
Figure 3
Values of a comet parameter (tail moment) for cells exposed to millimeter-wavelength 60 GHz radiation for 24 h: HCE-T (a) and SRA01/04 (b). The positive control was treatment with bleomycin (10 µg/mL). Data are presented as the mean ± SD from three independent experiments. Asterisks ** indicate p < 0.01. f = 0.1, (1−β) = 0.2193.
Figure 4
Figure 4
The expression of Hsp27 (a); Hsp70 (b) and Hsp90α (c) in HCE-T cells exposed to 60 GHz radiation for 24 h. The positive control underwent heat treatment at 43 °C for 2 h. Data are presented as the mean ± SD from three independent experiments. Asterisks ** indicate p < 0.01.
Figure 5
Figure 5
The expression of Hsp27 (a); Hsp70 (b) and Hsp90α (c) in SRA01/04 cells exposed to 60 GHz radiation for 24 h. The positive control underwent heat treatment at 43 °C for 30 min (Hsp27), 2 h (Hsp70) and 3 h (Hsp90α). Data are presented as the mean ± SD from three independent experiments. Asterisks * and ** indicate p < 0.05, and 0.01, respectively.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Beneduci A., Chidichimo G., De Rose R., Filippelli L., Straface S.V., Venuta S. Frequency and irradiation time-dependent antiprolifirative effect of low-power millimetre waves on RPMI 7932 human melanoma cell line. Anticancer Res. 2005;25:1023–1028. - PubMed
    1. Korenstein-Ilan A., Barbul A., Hasin P., Eliran A., Gover A., Korenstein R. Terahertz radiation increases genomic instability in human lymphocytes. Radiat. Res. 2008;170:224–234. doi: 10.1667/RR0944.1. - DOI - PubMed
    1. Alexandrov B.S., Rasmussen K.Ø., Bishop A.R., Usheva A., Alexandrov L.B., Chong S., Dagon Y., Booshehri L.G., Mielke C.H., Phipps M.L., et al. Non-thermal effects of terahertz radiation on gene expression in mouse stem cells. Biomed. Opt. Express. 2011;2:2679–2689. doi: 10.1364/BOE.2.002679. - DOI - PMC - PubMed
    1. Hintzsche H., Jastrow C., Kleine-Ostmann T., Stopper H., Schmid E., Schrader T. Terahertz radiation induces spindle disturbances in human-hamster hybrid cells. Radiat. Res. 2011;175:569–574. doi: 10.1667/RR2406.1. - DOI - PubMed
    1. Scarfì M.R., Romanò M., Di Pietro R., Zeni O., Doria A., Gallerano G.P., Giovenale E., Messina G., Lai A., Campurra G., et al. THz exposure of whole blood for the study of biological effects on human lymphocytes. J. Biol. Phys. 2003;29:171–176. doi: 10.1023/A:1024440708943. - DOI - PMC - PubMed