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Link to original content: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC267439
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. 1989 May;27(5):832–836. doi: 10.1128/jcm.27.5.832-836.1989

Detection of influenza C virus by using an in situ esterase assay.

P C Wagaman 1, H A Spence 1, R J O'Callaghan 1
PMCID: PMC267439  PMID: 2745694

Abstract

A variety of chemically defined compounds were tested to characterize the substrate specificity of the influenza C virus esterase and to determine whether a substrate could be found that would be useful in an assay to detect the virus. Two new substrates, alpha-naphthyl acetate and alpha-naphthyl propionate, were identified; alpha-naphthyl acetate was employed to develop an assay specific for influenza type C virus in MDCK cells. The assay was sufficiently sensitive to detect esterase activity in a single cell and distinguished influenza C virus infections from those of types A and B viruses. Infected cells could be detected as early as 8 h postinfection, with maximal enzyme detection occurring at 24 h. Assay of influenza C virus in the chorioallantoic or amniotic fluid of infected eggs was performed by applying fluids directly onto nitrocellulose strips and then incubating with alpha-naphthyl acetate. Both the cellular and nitrocellulose-bound assays are rapid, inexpensive, and easy to perform, offering advantages for use in clinical laboratories.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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