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Link to original content: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1358991
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Transactions of the American Ophthalmological Society logoLink to Transactions of the American Ophthalmological Society
. 2003;101:217–222.

Iris melanocyte numbers in Asian, African American, and Caucasian irides.

Daniel M Albert 1, W Richard Green 1, Michele L Zimbric 1, Cecilia Lo 1, Ronald E Gangnon 1, Kirsten L Hope 1, Joel Gleiser 1
PMCID: PMC1358991  PMID: 14971580

Abstract

PURPOSE: The anatomical basis for iris color has long been a controversial issue in ophthalmology. Recent studies demonstrated that in Caucasians, blue-eyed, gray-eyed, and hazel-eyed individuals have comparable numbers of iris melanocytes. The present investigation was carried out to compare melanocyte numbers in the irides of Asian, African American, and Caucasian brown-eyed individuals. METHODS: Paraffin-embedded sections from 71 brown-colored irides were incubated with rabbit anti-cow antibody against S100a, linked with an FITC conjugate antibody, and counterstained with Evans blue. Cells were counted under a fluorescence microscope and scored as melanocytes or other cells. Cell number, density, and iris area were calculated for each specimen. RESULTS: Caucasian and African American irides had comparable mean total melanocyte numbers. Asian irides had fewer total melanocytes than African American (P = .042) and Caucasian (P = .001) irides and smaller total number of cells (ie, melanocytes plus other cells) than African American (P = .054) or Caucasian (P = .009) irides. CONCLUSIONS: There is a statistically significant smaller mean total melanocyte number and mean total cellularity in Asian irides as compared to Caucasian and African American irides. This difference appears to be due to the combination of smaller iris area and lower melanocyte density in the Asian irides. The possibility exists that this may be a factor in ethnic variations in certain ocular diseases.

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Selected References

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  1. Chang T. S., Hay D., Courtright P. Age-related macular degeneration in Chinese-Canadians. Can J Ophthalmol. 1999 Aug;34(5):266–271. [PubMed] [Google Scholar]
  2. Congdon N. G., Foster P. J., Wamsley S., Gutmark J., Nolan W., Seah S. K., Johnson G. J., Broman A. T. Biometric gonioscopy and the effects of age, race, and sex on the anterior chamber angle. Br J Ophthalmol. 2002 Jan;86(1):18–22. doi: 10.1136/bjo.86.1.18. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Congdon N. G., Youlin Q., Quigley H., Hung P. T., Wang T. H., Ho T. C., Tielsch J. M. Biometry and primary angle-closure glaucoma among Chinese, white, and black populations. Ophthalmology. 1997 Sep;104(9):1489–1495. doi: 10.1016/s0161-6420(97)30112-2. [DOI] [PubMed] [Google Scholar]
  4. Dieterich C. E. Zur Feinstruktur der Melanocyten in der menschlichen Iris. Albrecht Von Graefes Arch Klin Exp Ophthalmol. 1972;183(4):317–333. doi: 10.1007/BF00496159. [DOI] [PubMed] [Google Scholar]
  5. Dutton J. J., Anderson R. L., Schelper R. L., Purcell J. J., Tse D. T. Orbital malignant melanoma and oculodermal melanocytosis: report of two cases and review of the literature. Ophthalmology. 1984 May;91(5):497–507. doi: 10.1016/s0161-6420(84)34261-0. [DOI] [PubMed] [Google Scholar]
  6. Eagle R. C., Jr Iris pigmentation and pigmented lesions: an ultrastructural study. Trans Am Ophthalmol Soc. 1988;86:581–687. [PMC free article] [PubMed] [Google Scholar]
  7. Eagle R. C., Jr Iris pigmentation and pigmented lesions: an ultrastructural study. Trans Am Ophthalmol Soc. 1988;86:581–687. [PMC free article] [PubMed] [Google Scholar]
  8. Foster P. J., Oen F. T., Machin D., Ng T. P., Devereux J. G., Johnson G. J., Khaw P. T., Seah S. K. The prevalence of glaucoma in Chinese residents of Singapore: a cross-sectional population survey of the Tanjong Pagar district. Arch Ophthalmol. 2000 Aug;118(8):1105–1111. doi: 10.1001/archopht.118.8.1105. [DOI] [PubMed] [Google Scholar]
  9. Gaudio P. A., Kaye D. B., Crawford J. Brooks. Histopathology of birdshot retinochoroidopathy. Br J Ophthalmol. 2002 Dec;86(12):1439–1441. doi: 10.1136/bjo.86.12.1439. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gonder J. R., Shields J. A., Albert D. M., Augsburger J. J., Lavin P. T. Uveal malignant melanoma associated with ocular and oculodermal melanocytosis. Ophthalmology. 1982 Aug;89(8):953–960. doi: 10.1016/s0161-6420(82)34694-1. [DOI] [PubMed] [Google Scholar]
  11. Gonder J. R., Shields J. A., Albert D. M., Augsburger J. J., Lavin P. T. Uveal malignant melanoma associated with ocular and oculodermal melanocytosis. Ophthalmology. 1982 Aug;89(8):953–960. doi: 10.1016/s0161-6420(82)34694-1. [DOI] [PubMed] [Google Scholar]
  12. Hiller R., Sperduto R. D., Krueger D. E. Race, iris pigmentation, and intraocular pressure. Am J Epidemiol. 1982 May;115(5):674–683. doi: 10.1093/oxfordjournals.aje.a113350. [DOI] [PubMed] [Google Scholar]
  13. Holz F. G., Piguet B., Minassian D. C., Bird A. C., Weale R. A. Decreasing stromal iris pigmentation as a risk factor for age-related macular degeneration. Am J Ophthalmol. 1994 Jan 15;117(1):19–23. doi: 10.1016/s0002-9394(14)73010-7. [DOI] [PubMed] [Google Scholar]
  14. Imesch P. D., Bindley C. D., Khademian Z., Ladd B., Gangnon R., Albert D. M., Wallow I. H. Melanocytes and iris color. Electron microscopic findings. Arch Ophthalmol. 1996 Apr;114(4):443–447. doi: 10.1001/archopht.1996.01100130439015. [DOI] [PubMed] [Google Scholar]
  15. Imesch P. D., Bindley C. D., Khademian Z., Ladd B., Gangnon R., Albert D. M., Wallow I. H. Melanocytes and iris color. Electron microscopic findings. Arch Ophthalmol. 1996 Apr;114(4):443–447. doi: 10.1001/archopht.1996.01100130439015. [DOI] [PubMed] [Google Scholar]
  16. Kahn H. J., Marks A., Thom H., Baumal R. Role of antibody to S100 protein in diagnostic pathology. Am J Clin Pathol. 1983 Mar;79(3):341–347. doi: 10.1093/ajcp/79.3.341. [DOI] [PubMed] [Google Scholar]
  17. Kan-Mitchell J., Rao N., Albert D. M., Van Eldik L. J., Taylor C. R. S100 immunophenotypes of uveal melanomas. Invest Ophthalmol Vis Sci. 1990 Aug;31(8):1492–1496. [PubMed] [Google Scholar]
  18. Kliman G. H., Augsburger J. J., Shields J. A. Association between iris color and iris melanocytic lesions. Am J Ophthalmol. 1985 Oct 15;100(4):547–548. doi: 10.1016/0002-9394(85)90679-8. [DOI] [PubMed] [Google Scholar]
  19. Rootman J., Gallagher R. P. Color as a risk factor in iris melanoma. Am J Ophthalmol. 1984 Nov;98(5):558–561. doi: 10.1016/0002-9394(84)90240-x. [DOI] [PubMed] [Google Scholar]
  20. Sandberg M. A., Gaudio A. R., Miller S., Weiner A. Iris pigmentation and extent of disease in patients with neovascular age-related macular degeneration. Invest Ophthalmol Vis Sci. 1994 May;35(6):2734–2740. [PubMed] [Google Scholar]
  21. Takahashi K., Isobe T., Ohtsuki Y., Akagi T., Sonobe H., Okuyama T. Immunohistochemical study on the distribution of alpha and beta subunits of S-100 protein in human neoplasm and normal tissues. Virchows Arch B Cell Pathol Incl Mol Pathol. 1984;45(4):385–396. doi: 10.1007/BF02889881. [DOI] [PubMed] [Google Scholar]
  22. Uyama M., Takahashi K., Ida N., Miyashiro M., Ando A., Takahashi A., Yamada E., Shirasu J., Nagai Y., Takeuchi M. The second eye of Japanese patients with unilateral exudative age related macular degeneration. Br J Ophthalmol. 2000 Sep;84(9):1018–1023. doi: 10.1136/bjo.84.9.1018. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wang Ningli, Wu Heping, Fan Zhigang. Primary angle closure glaucoma in Chinese and Western populations. Chin Med J (Engl) 2002 Nov;115(11):1706–1715. [PubMed] [Google Scholar]
  24. Wilkerson C. L., Syed N. A., Fisher M. R., Robinson N. L., Wallow I. H., Albert D. M. Melanocytes and iris color. Light microscopic findings. Arch Ophthalmol. 1996 Apr;114(4):437–442. doi: 10.1001/archopht.1996.01100130433014. [DOI] [PubMed] [Google Scholar]
  25. Wilkerson C. L., Syed N. A., Fisher M. R., Robinson N. L., Wallow I. H., Albert D. M. Melanocytes and iris color. Light microscopic findings. Arch Ophthalmol. 1996 Apr;114(4):437–442. doi: 10.1001/archopht.1996.01100130433014. [DOI] [PubMed] [Google Scholar]
  26. Wobmann P. R., Fine B. S. The clump cells of Koganei. A light and electron microscopic study. Am J Ophthalmol. 1972 Jan;73(1):90–101. doi: 10.1016/0002-9394(72)90311-x. [DOI] [PubMed] [Google Scholar]

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