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Link to original content: https://www.nature.com/nprot.rss
Nature Protocols Nature Protocols is an interactive online resource for laboratory protocols, providing step-by-step instructions for using and adapting research techniques that users can take straight to the lab bench and apply in their own research. Protocols are commissioned by the editorial team from leading laboratories. They are edited and peer-reviewed to ensure the highest level of quality and reproducibility. All protocols must have been proven to work, having been used to acquire data in published research papers. The focus is on providing practical information that is not available in research papers, such as explaining the critical points in the procedure, anticipated results (what to expect if the experiment has worked) and how to troubleshoot problems. Nature Protocols publishes protocols used to answer outstanding biological and biomedical research questions, including methods grounded in physics and chemistry that can be applied to biological problems. Protocols are added weekly and cover new methods, as well as classic, wellestablished techniques. Protocols are fully searchable online and also available in print on demand. http://feeds.nature.com/nprot/rss/current Nature Publishing Group en © 2024 Macmillan Publishers Limited, part of Springer Nature. All rights reserved. Nature Protocols © 2024 Macmillan Publishers Limited, part of Springer Nature. All rights reserved. permissions@nature.com Nature Protocols https://www.nature.com/uploads/product/nprot/rss.PNG http://feeds.nature.com/nprot/rss/current <![CDATA[ChromEMT: visualizing and reconstructing chromatin ultrastructure and 3D organization in situ]]> https://www.nature.com/articles/s41596-024-01071-2 Nature Protocols, Published online: 29 November 2024; doi:10.1038/s41596-024-01071-2

ChromEMT combines a fluorescent DNA binding dye that selectively enhances DNA and nucleosomes in electron microscopy with multi-tilt tomography, to enable the imaging and reconstruction of nuclear chromatin ultrastructure and 3D organization.]]> Horng D. OuSebastien PhanThomas J. DeerinckAkiko InagakiMark H. EllismanClodagh C. O’Shea doi:10.1038/s41596-024-01071-2 Nature Protocols, Published online: 2024-11-29; | doi:10.1038/s41596-024-01071-2 2024-11-29 Nature Protocols 10.1038/s41596-024-01071-2 https://www.nature.com/articles/s41596-024-01071-2 <![CDATA[Synthesis of chiral gold helicoid nanoparticles using glutathione]]> https://www.nature.com/articles/s41596-024-01083-y Nature Protocols, Published online: 25 November 2024; doi:10.1038/s41596-024-01083-y

The facile synthesis of the 432 helicoid III nanoparticle morphology, displaying a Kuhn’s dissymmetry factor (g-factor) of 0.2, is detailed by using l-glutathione in an aqueous solution.]]> Sang Won ImRyeong Myeong KimJeong Hyun HanIn Han HaHye-Eun LeeHyo-Yong AhnEunjeong JoKi Tae Nam doi:10.1038/s41596-024-01083-y Nature Protocols, Published online: 2024-11-25; | doi:10.1038/s41596-024-01083-y 2024-11-25 Nature Protocols 10.1038/s41596-024-01083-y https://www.nature.com/articles/s41596-024-01083-y <![CDATA[Author Correction: Creating custom synthetic genomes in <i>Escherichia coli</i> with REXER and GENESIS]]> https://www.nature.com/articles/s41596-024-01114-8 Nature Protocols, Published online: 21 November 2024; doi:10.1038/s41596-024-01114-8

Author Correction: Creating custom synthetic genomes in Escherichia coli with REXER and GENESIS]]> Escherichia coli with REXER and GENESIS]]> Wesley E. RobertsonLouise F. H. FunkeDaniel de la TorreJulius FredensKaihang WangJason W. Chin doi:10.1038/s41596-024-01114-8 Nature Protocols, Published online: 2024-11-21; | doi:10.1038/s41596-024-01114-8 2024-11-21 Nature Protocols 10.1038/s41596-024-01114-8 https://www.nature.com/articles/s41596-024-01114-8 <![CDATA[Biolayer interferometry for measuring the kinetics of protein–protein interactions and nanobody binding]]> https://www.nature.com/articles/s41596-024-01079-8 Nature Protocols, Published online: 21 November 2024; doi:10.1038/s41596-024-01079-8

Kinetic rates of protein–protein interactions can be measured with high throughput via biolayer interferometry.]]> Timothy A. BatesSintayehu K. GurmessaJules B. WeinsteinMila Trank-GreeneXammy Huu WrynlaAidan AnastasTeketay Wassie AnleyAudrey HinchliffUjwal ShindeJohn E. BurkeFikadu G. Tafesse doi:10.1038/s41596-024-01079-8 Nature Protocols, Published online: 2024-11-21; | doi:10.1038/s41596-024-01079-8 2024-11-21 Nature Protocols 10.1038/s41596-024-01079-8 https://www.nature.com/articles/s41596-024-01079-8 <![CDATA[RNA sample optimization for cryo-EM analysis]]> https://www.nature.com/articles/s41596-024-01072-1 Nature Protocols, Published online: 15 November 2024; doi:10.1038/s41596-024-01072-1

This protocol describes the optimization of RNA preparation conditions for cryo-EM structure determination, along with cryo-EM processing pipelines to resolve RNA dynamics and conformational changes, and workflows to generate moderate- to high-resolution cryo-EM density maps.]]> Xingyu ChenLiu WangJiahao XieJakub S. NowakBingnan LuoChong ZhangGuowen JiaJian ZouDingming HuangSebastian GlattYang YangZhaoming Su doi:10.1038/s41596-024-01072-1 Nature Protocols, Published online: 2024-11-15; | doi:10.1038/s41596-024-01072-1 2024-11-15 Nature Protocols 10.1038/s41596-024-01072-1 https://www.nature.com/articles/s41596-024-01072-1 <![CDATA[High-throughput glycosaminoglycan extraction and UHPLC-MS/MS quantification in human biofluids]]> https://www.nature.com/articles/s41596-024-01078-9 Nature Protocols, Published online: 14 November 2024; doi:10.1038/s41596-024-01078-9

We present a protocol detailing the extraction and quantification of glycosaminoglycans extracted from biofluids by using ultra-high-performance liquid chromatography combined with triple-quadrupole mass spectrometry.]]> Nicola VolpiFabio GaleottiFrancesco Gatto doi:10.1038/s41596-024-01078-9 Nature Protocols, Published online: 2024-11-14; | doi:10.1038/s41596-024-01078-9 2024-11-14 Nature Protocols 10.1038/s41596-024-01078-9 https://www.nature.com/articles/s41596-024-01078-9 <![CDATA[Versatile synthesis of uniform mesoporous superparticles from stable monomicelle units]]> https://www.nature.com/articles/s41596-024-01073-0 Nature Protocols, Published online: 13 November 2024; doi:10.1038/s41596-024-01073-0

This protocol presents a method for generating mesoporous superparticles from monomicelle units, with precise control of the number of the monomicelle units and the derived mesopores for superparticles.]]> Zaiwang ZhaoPengfei ZhangYujuan ZhaoLipeng WangJie ZhangFanxing BuWanhai ZhouRuizheng ZhaoXingmiao ZhangZirui LvYupu LiuYuan XiaWei ZhangTiancong ZhaoDongliang ChaoWei LiDongyuan Zhao doi:10.1038/s41596-024-01073-0 Nature Protocols, Published online: 2024-11-13; | doi:10.1038/s41596-024-01073-0 2024-11-13 Nature Protocols 10.1038/s41596-024-01073-0 https://www.nature.com/articles/s41596-024-01073-0 <![CDATA[Using the Chemotion repository to deposit and access FAIR research data for chemistry experiments]]> https://www.nature.com/articles/s41596-024-01074-z Nature Protocols, Published online: 08 November 2024; doi:10.1038/s41596-024-01074-z

Using the Chemotion repository to deposit and access FAIR research data for chemistry experiments]]> Pei-Chi HuangChia-Lin LinPierre TremouilhacNicole JungStefan Bräse doi:10.1038/s41596-024-01074-z Nature Protocols, Published online: 2024-11-08; | doi:10.1038/s41596-024-01074-z 2024-11-08 Nature Protocols 10.1038/s41596-024-01074-z https://www.nature.com/articles/s41596-024-01074-z