iBet uBet web content aggregator. Adding the entire web to your favor.
iBet uBet web content aggregator. Adding the entire web to your favor.



Link to original content: https://www.nature.com/articles/260500a0
Complete nucleotide sequence of bacteriophage MS2 RNA: primary and secondary structure of the replicase gene | Nature
Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Complete nucleotide sequence of bacteriophage MS2 RNA: primary and secondary structure of the replicase gene

Nature volume 260, pages 500–507 (1976)Cite this article

An Erratum to this article was published on 01 April 1976

Abstract

Bacteriophage MS2 RNA is 3,569 nucleotides long. The nucleotide sequence has been established for the third and last gene, which codes for the replicase protein. A secondary structure model has also been proposed. Biological properties, such as ribosome binding and codon interactions can now be discussed on a molecular basis. As the sequences for the other regions of this RNA have been published already, the complete, primary chemical structure of a viral genome has now been established.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on SpringerLink
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Zinder, N., (ed.) RNA Phage Book, 4, 353–396 (Cold Spring Harbor Laboratory, New York, 1975).

  2. Argetsinger-Steitz, J., J. molec. Biol., 33, 937–945 (1968).

    Article  Google Scholar 

  3. Weissmann, C., Billeter, M. A., Goodman, H. M., Hindley, J., and Weber, H., A. Rev. Biochem., 42, 303–328 (1973).

    Article  CAS  Google Scholar 

  4. Fedoroff, N. V., and Zinder, N. D., Proc. natn. Acad. Sci. U.S.A., 68, 1838–1843 (1971).

    Article  ADS  CAS  Google Scholar 

  5. Haruna, I., Nozu, K., Ohtaka, Y., and Spiegelman, S., Proc. natn. Acad. Sci. U.S.A., 50, 905–911 (1963).

    Article  ADS  CAS  Google Scholar 

  6. Weissmann, C., Simon, L., and Ochoa, S., Proc. natn. Acad. Sci. U.S.A., 49, 407–414 (1963).

    Article  ADS  CAS  Google Scholar 

  7. Kamen, R., Nature, 228, 527–533 (1970).

    Article  ADS  CAS  Google Scholar 

  8. Kondo, M., Gallerani, R., and Weissmann, C., Nature, 228, 525–527 (1970).

    Article  ADS  CAS  Google Scholar 

  9. Wahba, A. J., et al., J. biol. Chem., 249, 3314–3316 (1974).

    CAS  PubMed  Google Scholar 

  10. Kozak, M., and Nathans, D., Bact. Rev., 36, 109–134 (1972).

    CAS  Google Scholar 

  11. Fiers, W., et al., Nature, 256, 273–278 (1975).

    Article  ADS  CAS  Google Scholar 

  12. Kolakofsky, D., and Weissmann, C., Nature new Biol., 231, 42–46 (1971).

    Article  CAS  Google Scholar 

  13. Fiers, W., et al., Biochimie, 53, 495–506 (1971).

    Article  CAS  Google Scholar 

  14. Min Jou, W., Haegeman, G., Ysebaert, M., and Fiers, W., Nature, 237, 82–88 (1972).

    Article  ADS  CAS  Google Scholar 

  15. De Wachter, R., Merregaert, J., Vandenberghe, A., Contreras, R., and Fiers, W., Eur. J. Biochem., 22, 400–414 (1971).

    Article  CAS  Google Scholar 

  16. Fiers, W., in RNA Phage Book (edit. by Zinder, N.), 353–396 (Cold Spring Harbor Laboratory, New York, 1975).

    Google Scholar 

  17. Nichols, J. L., Nature, 225, 147–151 (1970).

    Article  ADS  CAS  Google Scholar 

  18. Vandenberghe, A., Min Jou, W., and Fiers, W., Proc. natn. Acad. Sci. U.S.A., 72, 2559–2562 (1975).

    Article  ADS  CAS  Google Scholar 

  19. Contreras, R., Vandenberghe, A., Volckaert, G., Min Jou, W., and Fiers, W., FEBS Lett., 24, 339–342 (1972).

    Article  CAS  Google Scholar 

  20. Rensing, U. F. E., Coulson, A., and Thompson, E. O. P., Biochem. J., 131, 605–610 (1973).

    Article  CAS  Google Scholar 

  21. De Wachter, R., and Fiers, W., Analyt. Biochem., 49, 184–197 (1972).

    Article  CAS  Google Scholar 

  22. Sanger, F., Brownlee, G. G., and Barrell, B. G., J. molec. Biol., 13, 373–398 (1965).

    Article  CAS  Google Scholar 

  23. Brownlee, G. G., Determination of Sequences in RNA (edit. by Work, T. S., and Work, E.) (North-Holland, Amsterdam, 1972).

    Book  Google Scholar 

  24. Haegeman, G., and Fiers, W., Eur. J. Biochem., 36, 135–143 (1973).

    Article  CAS  Google Scholar 

  25. Volckaert, G., and Fiers, W., Analyt. Biochem., 62, 573–583 (1974).

    Article  CAS  Google Scholar 

  26. Contreras, R., and Fiers, W., Analyt. Biochem., 67, 319–326 (1975).

    Article  CAS  Google Scholar 

  27. Contreras, R., and Fiers, W., FEBS Lett., 16, 281–283 (1971).

    Article  CAS  Google Scholar 

  28. Volckaert, G., Min Jou, W., and Fiers, W., Analyt. Biochem. (in the press).

  29. Tinoco, I., Jr, et al., Nature new biol., 246, 40–41 (1973).

    Article  CAS  Google Scholar 

  30. Borer, P. N., Dengler, B., and Tinoco, I., Jr, J. molec. Biol., 86, 843–853 (1974).

    Article  CAS  Google Scholar 

  31. Boedtker, H., Biochemistry, 6, 2718–2727 (1967).

    Article  CAS  Google Scholar 

  32. Siegers, H., Clauwaert, J., and Fiers, W., Biopolymers, 12, 2033–2044 (1973).

    Article  Google Scholar 

  33. Gralla, J., Steitz, J. A., and Crothers, D. M., Nature, 248, 204–208 (1974).

    Article  ADS  CAS  Google Scholar 

  34. Klug, A., Ladner, J., and Robertus, J. D., J. molec. Biol., 89, 511–516 (1974).

    Article  CAS  Google Scholar 

  35. Lodish, H. F., Nature, 220, 345–349 (1968).

    Article  ADS  CAS  Google Scholar 

  36. Osborn, M., Weber, K., and Lodish, H. F., Biochem. biophys. Res. Commun., 41, 748–756 (1970).

    Article  CAS  Google Scholar 

  37. Argetsinger-Steitz, J., Nature, 224, 957–964 (1969).

    Article  Google Scholar 

  38. Shine, J., and Dalgarno, L., Nature, 254, 34–38 (1975).

    Article  ADS  CAS  Google Scholar 

  39. Argetsinger-Steitz, J., and Jakes, K., Proc. natn. Acad. Sci. U.S.A., 72, 4734–4738 (1975).

    Article  ADS  Google Scholar 

  40. Lodish, H. F., J. molec. Biol., 50, 689–702 (1970).

    Article  CAS  Google Scholar 

  41. Argetsinger-Steitz, J., J. molec. Biol., 73, 1–16 (1973).

    Article  Google Scholar 

  42. Bernardi, A., and Spahr, P. F., Proc. natn. Acad. Sci. U.S.A., 69, 3033–3037 (1972).

    Article  ADS  CAS  Google Scholar 

  43. Atkins, J. F., and Gesteland, R. F., Molec. gen. Genet., 139, 19–31 (1975).

    Article  CAS  Google Scholar 

  44. Senear, A. W., and Argetsinger-Steitz, J., J. biol. Chem. (in the press).

  45. Fedoroff, N. V., and Zinder, N. D., Nature new Biol., 241, 105–108 (1973).

    Article  CAS  Google Scholar 

  46. Carmichael, G. G., Weber, K., Niveleau, A., and Wahba, A. J., J. biol. Chem., 250, 3607–3612 (1975).

    CAS  PubMed  Google Scholar 

  47. Min Jou, W., Haegeman, G., and Fiers, W., FEBS Lett., 13, 105–109 (1971).

    Article  CAS  Google Scholar 

  48. Adams, J. M., Jeppesen, P. G. N., Sanger, F., and Barrell, B. G., Nature, 223, 1009–1014 (1969).

    Article  ADS  CAS  Google Scholar 

  49. Fitch, W. M., J. molec. Biol., 3, 279–291 (1974).

    ADS  CAS  Google Scholar 

  50. Gralla, J., and Delisi, C., Nature, 248, 330–332 (1974).

    Article  ADS  CAS  Google Scholar 

  51. Ricard, B., and Salser, W., Biochem. biophys. Res. Commun., 63, 548–554 (1975).

    Article  CAS  Google Scholar 

  52. Air, G. M., Blackburn, E. H., Sanger, F., and Coulson, A. R., J. molec. Biol., 96, 703–719 (1975).

    Article  CAS  Google Scholar 

  53. Harada, F., and Nishimura, S., Biochemistry, 13, 300–307 (1974).

    Article  CAS  Google Scholar 

  54. Fiers, W., et al., Abstr. 3rd int. Cong. Virol., Madrid, 17 (1975).

  55. Weiner, A. M., and Weber, K., Nature new Biol., 234, 206–209 (1971).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Molecular Biology, University of Ghent, 9000, Ghent, Belgium

    W. Fiers, R. Contreras, F. Duerinck, G. Haegeman, D. Iserentant, J. Merregaert, W. Min Jou, F. Molemans, A. Raeymaekers, A. Van den Berghe, G. Volckaert & M. Ysebaert

Authors
  1. W. Fiers
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. Contreras
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. F. Duerinck
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. G. Haegeman
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. D. Iserentant
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. J. Merregaert
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. W. Min Jou
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. F. Molemans
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. A. Raeymaekers
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. A. Van den Berghe
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. G. Volckaert
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. M. Ysebaert
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fiers, W., Contreras, R., Duerinck, F. et al. Complete nucleotide sequence of bacteriophage MS2 RNA: primary and secondary structure of the replicase gene. Nature 260, 500–507 (1976). https://doi.org/10.1038/260500a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/260500a0

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing