iBet uBet web content aggregator. Adding the entire web to your favor.
iBet uBet web content aggregator. Adding the entire web to your favor.



Link to original content: https://pubmed.ncbi.nlm.nih.gov/6270629
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches - PubMed Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1981 Aug;391(2):85-100.
doi: 10.1007/BF00656997.

Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches

O P Hamill, A Marty, E Neher, B Sakmann, F J Sigworth

Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches

O P Hamill et al. Pflugers Arch. 1981 Aug.

Abstract

1. The extracellular patch clamp method, which first allowed the detection of single channel currents in biological membranes, has been further refined to enable higher current resolution, direct membrane patch potential control, and physical isolation of membrane patches. 2. A description of a convenient method for the fabrication of patch recording pipettes is given together with procedures followed to achieve giga-seals i.e. pipette-membrane seals with resistances of 10(9) - 10(11) omega. 3. The basic patch clamp recording circuit, and designs for improved frequency response are described along with the present limitations in recording the currents from single channels. 4. Procedures for preparation and recording from three representative cell types are given. Some properties of single acetylcholine-activated channels in muscle membrane are described to illustrate the improved current and time resolution achieved with giga-seals. 5. A description is given of the various ways that patches of membrane can be physically isolated from cells. This isolation enables the recording of single channel currents with well-defined solutions on both sides of the membrane. Two types of isolated cell-free patch configurations can be formed: an inside-out patch with its cytoplasmic membrane face exposed to the bath solution, and an outside-out patch with its extracellular membrane face exposed to the bath solution. 6. The application of the method for the recording of ionic currents and internal dialysis of small cells is considered. Single channel resolution can be achieved when recording from whole cells, if the cell diameter is small (less than 20 micrometer). 7. The wide range of cell types amenable to giga-seal formation is discussed.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. J Physiol. 1976 Dec;263(3):417-39 - PubMed
    1. Nature. 1980 Jul 3;286(5768):71-3 - PubMed
    1. Nature. 1980 Oct 2;287(5781):447-9 - PubMed
    1. J Gen Physiol. 1978 May;71(5):489-507 - PubMed
    1. Neuroscience. 1980;5(12):2325-7 - PubMed

Publication types

LinkOut - more resources