iBet uBet web content aggregator. Adding the entire web to your favor.
iBet uBet web content aggregator. Adding the entire web to your favor.



Link to original content: https://pubmed.ncbi.nlm.nih.gov/37218079/
Experimental evidence that lignin-modifying enzymes are essential for degrading plant cell wall lignin by Pleurotus ostreatus using CRISPR/Cas9 - PubMed Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Oct;25(10):1909-1924.
doi: 10.1111/1462-2920.16427. Epub 2023 May 22.

Experimental evidence that lignin-modifying enzymes are essential for degrading plant cell wall lignin by Pleurotus ostreatus using CRISPR/Cas9

Takehito Nakazawa  1 , Iori Yamaguchi  1 , Yufan Zhang  1 , Chinami Saka  1 , Hongli Wu  1 , Keita Kayama  1 , Moriyuki Kawauchi  1 , Masahiro Sakamoto  1 , Yoichi Honda  1
Affiliations

Experimental evidence that lignin-modifying enzymes are essential for degrading plant cell wall lignin by Pleurotus ostreatus using CRISPR/Cas9

Takehito Nakazawa et al. Environ Microbiol. 2023 Oct.

Abstract

Lignin-modifying enzymes (LMEs), which include laccases (Lacs), manganese peroxidases (MnPs), versatile peroxidases (VPs), and lignin peroxidases (LiPs), have been considered key factors in lignin degradation by white-rot fungi because they oxidize lignin model compounds and depolymerize synthetic lignin in vitro. However, it remains unclear whether these enzymes are essential/important in the actual degradation of natural lignin in plant cell walls. To address this long-standing issue, we examined the lignin-degrading abilities of multiple mnp/vp/lac mutants of Pleurotus ostreatus. One vp2/vp3/mnp3/mnp6 quadruple-gene mutant was generated from a monokaryotic wild-type strain PC9 using plasmid-based CRISPR/Cas9. Also, two vp2/vp3/mnp2/mnp3/mnp6, two vp2/vp3/mnp3/mnp6/lac2 quintuple-gene mutants, and two vp2/vp3/mnp2/mnp3/mnp6/lac2 sextuple-gene mutants were generated. The lignin-degrading abilities of the sextuple and vp2/vp3/mnp2/mnp3/mnp6 quintuple-gene mutants on the Beech wood sawdust medium reduced drastically, but not so much for those of the vp2/vp3/mnp3/mnp6/lac2 mutants and the quadruple mutant strain. The sextuple-gene mutants also barely degraded lignin in Japanese Cedar wood sawdust and milled rice straw. Thus, this study presented evidence that the LMEs, especially MnPs and VPs, play a crucial role in the degradation of natural lignin by P. ostreatus for the first time.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

REFERENCES

    1. Adinarayana, R. (1995) The potential for white-rot fungi in the treatment of pollutants. Current Opinion in Biotechniology, 6, 320-328. Available from: https://doi.org/10.1016/0958-1669(95)80054-9
    1. Arantes, V. & Goodell, B. (2014) Current understanding of brown-rot fungal biodegradation mechanisms: a review. In: Schultz, T.P., Goodell, B. & Nicholas, D.D. (Eds.) Deterioration and protection of sustainable biomaterials. Washington: ACS Publications, pp. 3-21. Available from: https://doi.org/10.1021/bk-2014-1158.ch001
    1. Boontawon, T., Nakazawa, T., Horii, M., Tsuzuki, M., Kawauchi, M., Sakamoto, M. et al. (2021) Functional analysis of Pleurotus ostreatus pcc1 and clp1 using CRISPR/Cas9. Fungal Genetics and Biology, 154, 103599. Available from: https://doi.org/10.1016/j.fgb.2021.103599
    1. Boontawon, T., Nakazawa, T., Inoue, C., Osakabe, K., Kawauchi, M., Sakamoto, M. et al. (2021) Efficient genome editing with CRISPR/Cas9 in Pleurotus ostreatus. AMB Express, 11, 30. Available from: https://doi.org/10.1186/s13568-021-01193-w
    1. Cragg, S.M., Beckham, G.T., Bruce, N.C., Bugg, T.D.H., Distel, D.L., Dupree, P. et al. (2015) Lignocellulose degradation mechanisms across the tree of life. Curr Opinion in Chemical Biology, 29, 108-119. Available from: https://doi.org/10.1016/j.cbpa.2015.10.018

Publication types

LinkOut - more resources