RACGAP1 modulates ECT2-Dependent mitochondrial quality control to drive breast cancer metastasis
- PMID: 33485843
- DOI: 10.1016/j.yexcr.2021.112493
RACGAP1 modulates ECT2-Dependent mitochondrial quality control to drive breast cancer metastasis
Abstract
Most cancer deaths are due to the colonization of tumor cells in distant organs. More evidence indicates that overexpression of RACGAP1 plays a critical role in cancer metastasis. However, the underlying mechanism still remains poorly understood. Here we found that RACGAP1 promoted breast cancer metastasis through regulating mitochondrial quality control. Overexpression of RACGAP1 in breast cancer cells led to the fragmentation of mitochondria, increased mitophagy intensity, mitochondrial turnover, and aerobic glycolysis ATP production. We showed that RACGAP1 promoted mitochondrial fission through recruiting ECT2 during anaphase and subsequently had activated ERK-DRP1 pathway. We further demonstrated the phosphorylation of RACGAP1 is essential for its ability of binding with ECT2 and its downstream effects. RACGAP1 overexpression also increased the expression of PGC-1a, a key mitochondrial biogenesis regulator, presumably by the increased mitophagy intensity induced by RACGAP1. PGC-1a increased the enrichment of DNMT1 in mitochondria, mitochondrial DNMT1 augmented mitochondrial DNA methylation and upregulated mitochondrial genome transcription. Our data indicated that RACGAP1 simultaneously facilitated mitophagy and mitochondrial biogenesis through regulating DRP1 phosphorylation and PGC-1a expression, eventually improved mitochondrial quality control in breast cancer cells. Our study provided a new angle in understanding the RACGAP1-overexpression related malignancy in breast cancer patients.
Keywords: Breast cancer; Mitochondrial dynamics; Mitochondrial quality control; RACGAP1; Tumor metastasis.
Copyright © 2021. Published by Elsevier Inc.
Similar articles
-
Overexpression of Rac GTPase Activating Protein 1 Contributes to Proliferation of Cancer Cells by Reducing Hippo Signaling to Promote Cytokinesis.Gastroenterology. 2018 Oct;155(4):1233-1249.e22. doi: 10.1053/j.gastro.2018.07.010. Epub 2018 Sep 5. Gastroenterology. 2018. PMID: 30009820
-
Long non-coding RNA RACGAP1P promotes breast cancer invasion and metastasis via miR-345-5p/RACGAP1-mediated mitochondrial fission.Mol Oncol. 2021 Feb;15(2):543-559. doi: 10.1002/1878-0261.12866. Epub 2020 Dec 16. Mol Oncol. 2021. PMID: 33252198 Free PMC article.
-
Polyphyllin I induces mitophagic and apoptotic cell death in human breast cancer cells by increasing mitochondrial PINK1 levels.Oncotarget. 2017 Feb 7;8(6):10359-10374. doi: 10.18632/oncotarget.14413. Oncotarget. 2017. PMID: 28060722 Free PMC article.
-
The regulation of tumor cell physiology by mitochondrial dynamics.Biochem Biophys Res Commun. 2018 May 27;500(1):9-16. doi: 10.1016/j.bbrc.2017.06.192. Epub 2017 Jul 1. Biochem Biophys Res Commun. 2018. PMID: 28676396 Free PMC article. Review.
-
Assessment of RACGAP1 as a Prognostic and Immunological Biomarker in Multiple Human Tumors: A Multiomics Analysis.Int J Mol Sci. 2022 Nov 15;23(22):14102. doi: 10.3390/ijms232214102. Int J Mol Sci. 2022. PMID: 36430577 Free PMC article. Review.
Cited by
-
Transcription factor ETS1‑mediated ECT2 expression promotes the malignant behavior of prostate cancer cells.Oncol Lett. 2024 Jul 23;28(4):453. doi: 10.3892/ol.2024.14585. eCollection 2024 Oct. Oncol Lett. 2024. PMID: 39100995 Free PMC article.
-
Integrating bioinformatics and machine learning methods to analyze diagnostic biomarkers for HBV-induced hepatocellular carcinoma.Diagn Pathol. 2024 Aug 2;19(1):105. doi: 10.1186/s13000-024-01528-8. Diagn Pathol. 2024. PMID: 39095799 Free PMC article.
-
RACGAP1 knockdown synergizes and enhances the effects of chemotherapeutics on ovarian cancer.Am J Transl Res. 2024 May 15;16(5):2132-2146. doi: 10.62347/QNZU1402. eCollection 2024. Am J Transl Res. 2024. PMID: 38883382 Free PMC article.
-
Epidemiological and transcriptome data identify shared gene signatures and immune cell infiltration in type 2 diabetes and non-small cell lung cancer.Diabetol Metab Syndr. 2024 Mar 12;16(1):64. doi: 10.1186/s13098-024-01278-z. Diabetol Metab Syndr. 2024. PMID: 38468345 Free PMC article.
-
RBFOX2 deregulation promotes pancreatic cancer progression and metastasis through alternative splicing.Nat Commun. 2023 Dec 19;14(1):8444. doi: 10.1038/s41467-023-44126-w. Nat Commun. 2023. PMID: 38114498 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Research Materials
Miscellaneous