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Link to original content: https://pubmed.ncbi.nlm.nih.gov/30972478
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. 2019 Apr;30(3-4):63-70.
doi: 10.1007/s00335-019-09799-z. Epub 2019 Apr 10.

A high-density genetic map and molecular sex-typing assay for gerbils

Thomas D Brekke  1 , Sushmita Supriya  1 , Megan G Denver  1 , Angharad Thom  1 , Katherine A Steele  1 , John F Mulley  2
Affiliations

A high-density genetic map and molecular sex-typing assay for gerbils

Thomas D Brekke et al. Mamm Genome. 2019 Apr.

Abstract

We constructed a high-density genetic map for Mongolian gerbils (Meriones unguiculatus). We genotyped 137 F2 individuals with a genotype-by-sequencing (GBS) approach at over 10,000 loci and built the genetic map using a two-step approach. First, we chose the highest-quality set of 485 markers to construct a robust map of 1239 cM with 22 linkage groups as expected from the published karyotype. Second, we added an additional 5449 markers onto the map based on their genotype similarity with the original markers. We used the final marker set to assemble 1140 genomic scaffolds (containing ~ 20% of annotated genes) into a chromosome-level assembly. We used both genetic linkage and relative sequencing coverage in males and females to identify X- and Y-chromosome scaffolds and from these we designed a robust and internally-controlled PCR assay to determine sex. This assay will facilitate early stage sex-typing of embryonic and young gerbils which is difficult using current visual methods. Accession ID: Meriones unguiculatus: 10047.

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Conflict of interest statement

On behalf of all authors, the corresponding author states that there is no conflict of interest.

Figures

Fig. 1
Fig. 1
Genetic map of the Mongolian gerbil genome. The map contains 6034 SNP markers, assigned to 21 autosomes and the X chromosome, and comprises 1239.1 cM
Fig. 2
Fig. 2
Sex-specific coverage of markers. Y-linked markers have no coverage in females, and X-linked markers have twice the coverage in females as males
Fig. 3
Fig. 3
PCR assay for sex-typing gerbils. DNA extracted from liver for both Mongolian gerbils (a) and fat sandrats (b) can be robustly amplified with the primer pair. Males amplify two bands while females amplify one. The Y-linked band is at 845 bases while the X-linked band is at 206 bp. The ladder is the Bioline 1 kb ladder. ‘Neg’ is the no-template control
See this image and copyright information in PMC

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