iBet uBet web content aggregator. Adding the entire web to your favor.
iBet uBet web content aggregator. Adding the entire web to your favor.



Link to original content: https://pubmed.ncbi.nlm.nih.gov/30768168
Characterization of the Cytochrome P450 Gene CYP305A1 of the Cotton Aphid (Hemiptera: Aphididae) and Its Responsive Cis-Elements to Plant Allelochemicals - PubMed Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 May 22;112(3):1365-1371.
doi: 10.1093/jee/toz021.

Characterization of the Cytochrome P450 Gene CYP305A1 of the Cotton Aphid (Hemiptera: Aphididae) and Its Responsive Cis-Elements to Plant Allelochemicals

Fen Li  1   2 , Kangsheng Ma  1 , Ying Liu  1 , Jing-Jiang Zhou  2   3 , Xiwu Gao  1
Affiliations

Characterization of the Cytochrome P450 Gene CYP305A1 of the Cotton Aphid (Hemiptera: Aphididae) and Its Responsive Cis-Elements to Plant Allelochemicals

Fen Li et al. J Econ Entomol. .

Abstract

Insect cytochrome P450 monooxygenases play an important role in plant allelochemical detoxification. In this study, a full-length gene CYP305A1 of the P450 Clan 2 family was cloned from Aphis gossypii Glover, and its promoter was identified and characterized. The transcript level of CYP305A1 and its promoter activity were significantly induced by two plant allelochemicals, gossypol and 2-tridecanone. Furthermore, the 5'-end promoter region from -810 to +62 bp was demonstrated to be essential for basal transcriptional activity of CYP305A1, and the promoter region from -810 to -581 bp was shown as an essential plant allelochemical responsive element and had a cis-element 5'-CACACTA-3' as the binding site of aryl hydrocarbon receptor. Interestingly, there was an identical overlapping region of 1,094 bp between CYP305A1 promoter and the venom protease gene. When the expression of CYP305A1 gene was knocked down by RNA interference with CYP305A1 dsRNA, the expression of the venom protease gene was decreased. However, the knockdown of the expression of the venom protease gene did not affect the CYP305A1 expression. These results provide important insights for understanding the functions of P450 genes and the regulatory mechanism of P450 gene expressions in the resistance of Aphis gossypii Glover to plant allelochemicals.

Keywords: Aphis gossypii; CYP305A1; plant allelochemical; promoter; venom protease.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

Publication types

LinkOut - more resources