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Link to original content: https://pubmed.ncbi.nlm.nih.gov/2860972/
Down-modulation of an oncogene protein product and reversion of the transformed phenotype by monoclonal antibodies - PubMed Skip to main page content
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. 1985 Jul;41(3):697-706.
doi: 10.1016/s0092-8674(85)80050-7.

Down-modulation of an oncogene protein product and reversion of the transformed phenotype by monoclonal antibodies

J A Drebin, V C Link, D F Stern, R A Weinberg, M I Greene

Down-modulation of an oncogene protein product and reversion of the transformed phenotype by monoclonal antibodies

J A Drebin et al. Cell. 1985 Jul.

Abstract

Exposure of neu-oncogene-transformed NIH 3T3 cells to monoclonal antibodies reactive with the neu gene product, p185, results in the rapid and reversible loss of both cell-surface and total cellular p185. Although not directly cytotoxic, monoclonal anti-p185 antibody treatment causes neu-transformed NIH 3T3 cells to revert to a nontransformed phenotype, as determined by anchorage-independent growth. Isotype matched control antibodies of an unrelated specificity do not affect p185 levels or colony formation in soft agar by neu-transformed NIH 3T3 cells. Soft agar colony formation by NIH 3T3 cells transformed by ras oncogenes is not affected by anti-p185 antibody treatment. Anchorage-independent growth of cells from the ethylnitrosourea-induced rat neuroblastoma line in which neu was originally detected by DNA transfection is also inhibited in the presence of anti-p185 monoclonal antibodies. Collectively, these results suggest that p185 is required to maintain transformation induced by the neu oncogene.

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