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Link to original content: https://pubmed.ncbi.nlm.nih.gov/27343839
Cross-reactivity between methylisothiazolinone, octylisothiazolinone and benzisothiazolinone using a modified local lymph node assay - PubMed Skip to main page content
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. 2017 Jan;176(1):176-183.
doi: 10.1111/bjd.14825. Epub 2016 Nov 26.

Cross-reactivity between methylisothiazolinone, octylisothiazolinone and benzisothiazolinone using a modified local lymph node assay

J F Schwensen  1 , C Menné Bonefeld  2 , C Zachariae  3 , C Agerbeck  2 , T H Petersen  2 , C Geisler  2 , U E Bollmann  4 , K Bester  4 , J D Johansen  1
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Cross-reactivity between methylisothiazolinone, octylisothiazolinone and benzisothiazolinone using a modified local lymph node assay

J F Schwensen et al. Br J Dermatol. 2017 Jan.

Abstract

Background: In the light of the exceptionally high rates of contact allergy to the preservative methylisothiazolinone (MI), information about cross-reactivity between MI, octylisothiazolinone (OIT) and benzisothiazolinone (BIT) is needed.

Objectives: To study cross-reactivity between MI and OIT, and between MI and BIT.

Methods: Immune responses to MI, OIT and BIT were studied in vehicle and MI-sensitized female CBA mice by a modified local lymph node assay. The inflammatory response was measured by ear thickness, cell proliferation of CD4+ and CD8+ T cells, and CD19+ B cells in the auricular draining lymph nodes.

Results: MI induced significant, strong, concentration-dependent immune responses in the draining lymph nodes following a sensitization phase of three consecutive days. Groups of MI-sensitized mice were challenged on day 23 with 0·4% MI, 0·7% OIT and 1·9% BIT - concentrations corresponding to their individual EC3 values. No statistically significant difference in proliferation of CD4+ and CD8+ T cells was observed between mice challenged with MI compared with mice challenged with BIT and OIT.

Conclusions: The data indicate cross-reactivity between MI, OIT and BIT, when the potency of the chemical was taken into account in choice of challenge concentration. This means that MI-sensitized individuals may react to OIT and BIT if exposed to sufficient concentrations.

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