iBet uBet web content aggregator. Adding the entire web to your favor.
iBet uBet web content aggregator. Adding the entire web to your favor.



Link to original content: https://pubmed.ncbi.nlm.nih.gov/26465384/
Molecular Evidence of Sexual Transmission of Ebola Virus - PubMed Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Case Reports
. 2015 Dec 17;373(25):2448-54.
doi: 10.1056/NEJMoa1509773. Epub 2015 Oct 14.

Molecular Evidence of Sexual Transmission of Ebola Virus

Suzanne E Mate  1 , Jeffrey R Kugelman, Tolbert G Nyenswah, Jason T Ladner, Michael R Wiley, Thierry Cordier-Lassalle, Athalia Christie, Gary P Schroth, Stephen M Gross, Gloria J Davies-Wayne, Shivam A Shinde, Ratnesh Murugan, Sonpon B Sieh, Moses Badio, Lawrence Fakoli, Fahn Taweh, Emmie de Wit, Neeltje van Doremalen, Vincent J Munster, James Pettitt, Karla Prieto, Ben W Humrighouse, Ute Ströher, Joseph W DiClaro, Lisa E Hensley, Randal J Schoepp, David Safronetz, Joseph Fair, Jens H Kuhn, David J Blackley, A Scott Laney, Desmond E Williams, Terrence Lo, Alex Gasasira, Stuart T Nichol, Pierre Formenty, Francis N Kateh, Kevin M De Cock, Fatorma Bolay, Mariano Sanchez-Lockhart, Gustavo Palacios
Affiliations
Case Reports

Molecular Evidence of Sexual Transmission of Ebola Virus

Suzanne E Mate et al. N Engl J Med. .

Abstract

A suspected case of sexual transmission from a male survivor of Ebola virus disease (EVD) to his female partner (the patient in this report) occurred in Liberia in March 2015. Ebola virus (EBOV) genomes assembled from blood samples from the patient and a semen sample from the survivor were consistent with direct transmission. The genomes shared three substitutions that were absent from all other Western African EBOV sequences and that were distinct from the last documented transmission chain in Liberia before this case. Combined with epidemiologic data, the genomic analysis provides evidence of sexual transmission of EBOV and evidence of the persistence of infective EBOV in semen for 179 days or more after the onset of EVD. (Funded by the Defense Threat Reduction Agency and others.).

PubMed Disclaimer

Figures

Figure 1
Figure 1. Clinical Timelines for the Patient and the Survivor, from September 2014 through May 2015
Shown are key dates regarding the Ebola virus disease (EVD) presentation, diagnostic tests, and outcomes for the survivor (S) and the patient (P). Horizontal bars estimate the number of days of persistence of the Ebola virus (EBOV) since the date of disease onset and since the date of clearance from blood. ETU denotes Ebola treatment unit.
Figure 2
Figure 2. Median-Joining Haplotype Network
This network was constructed from a full genome alignment of 100 clinical sequences of the Ebola virus Makona variant, including those assembled from blood samples obtained from the patient (P), the survivor’s older brother (SB), and the survivor’s former wife (SFW), from a semen sample from the survivor (S), and from 96 additional genomes chosen from the 796 genomes that were analyzed to be representative of samples collected in Guinea, Liberia, Mali, and Sierra Leone. For visual clarity, the network was limited to 100 genomes. The GenBank accession numbers for the tested genomes are as follows: for P, the number is KT587343, for S, the number is KT587344, for SB, the number is KT587346, and for SFW, the number is KT587345. Each colored vertex represents a sampled viral haplotype. The vertex size is proportional to the number of sampled sequences. Genomes sequenced in this study are shown in pink. Purple vertexes (SPB) indicate samples from the last known cluster of EVD cases in Liberia before the infection of the patient discussed in this report. Other colors indicate the respective countries of origin. Edges are not drawn to scale; hatch marks indicate the number of substitutions along each edge. The vertex SL2 represents the ancestral haplotype that is thought to have been introduced into Liberia in the spring of 2014.,
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Geneva: World Health Organization; 2015. Ebola situation reports. http://apps.who.int/ebola/ebola-situation-reports.
    1. Clark DV, Kibuuka H, Millard M, et al. Long-term sequelae after Ebola virus disease in Bundibugyo, Uganda: a retrospective cohort study. Lancet Infect Dis. 2015;15:905–912. - PubMed
    1. Bausch DG, Towner JS, Dowell SF, et al. Assessment of the risk of Ebola virus transmission from bodily fluids and fomites. J Infect Dis. 2007;196(Suppl 2):S142–S147. - PubMed
    1. Rodriguez LL, De Roo A, Guimard Y, et al. Persistence and genetic stability of Ebola virus during the outbreak in Kikwit, Democratic Republic of the Congo, 1995. J Infect Dis. 1999;179(Suppl 1):S170–S176. - PubMed
    1. Varkey JB, Shantha JG, Crozier I, et al. Persistence of Ebola virus in ocular fluid during convalescence. N Engl J Med. 2015;372:2423–2427. - PMC - PubMed

Publication types