Investigation of mtDNA control region sequences in an Egyptian population sample
- PMID: 23910099
- DOI: 10.1016/j.legalmed.2013.06.006
Investigation of mtDNA control region sequences in an Egyptian population sample
Abstract
The sequences of mitochondrial DNA (mtDNA) control region were investigated in 101 unrelated individuals living in the northern region of Nile delta (Gharbia, N=55 and Kafrelsheikh, N=46). DNA was extracted from blood stained filter papers or buccal swabs. HV1, HV2 and HV3 were PCR amplified and sequenced; the resulted sequences were aligned and compared with revised Cambridge sequence (rCRS). The results revealed presence of total 93 different haplotypes, 86 of them are unique and 7 are shared haplotypes, the most common haplotype, was observed with a frequency, 2.97% of population sample. High mtDNA diversity was observed with genetic diversity and power of discrimination, 0.9982 and 0.9883, respectively. In this dataset the west Eurasian haplogroups predominated over the African haplogroups. The results would be useful for forensic examinations and human genetic studies.
Keywords: Egypt delta; Forensic data; Hyper variable regions; Mitochondrial DNA.
Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.
Similar articles
-
Mitochondrial DNA control region polymorphism in the population of Alagoas state, north-eastern Brazil.J Forensic Sci. 2008 Jan;53(1):142-6. doi: 10.1111/j.1556-4029.2007.00619.x. J Forensic Sci. 2008. PMID: 18279250
-
Sequence polymorphisms of mtDNA HV1, HV2, and HV3 regions in the Malay population of Peninsular Malaysia.Int J Legal Med. 2010 Sep;124(5):415-26. doi: 10.1007/s00414-010-0469-x. Epub 2010 May 26. Int J Legal Med. 2010. PMID: 20502908
-
Sequence polymorphism of the mitochondrial DNA hypervariable regions I and II in 205 Singapore Malays.Leg Med (Tokyo). 2007 Jan;9(1):33-7. doi: 10.1016/j.legalmed.2006.08.007. Epub 2006 Dec 5. Leg Med (Tokyo). 2007. PMID: 17150401
-
Increasing the forensic discrimination of mitochondrial DNA testing through analysis of the entire mitochondrial DNA genome.Croat Med J. 2001 Jun;42(3):304-9. Croat Med J. 2001. PMID: 11387644 Review.
-
The case for the continuing use of the revised Cambridge Reference Sequence (rCRS) and the standardization of notation in human mitochondrial DNA studies.J Hum Genet. 2014 Feb;59(2):66-77. doi: 10.1038/jhg.2013.120. Epub 2013 Dec 5. J Hum Genet. 2014. PMID: 24304692 Review.
Cited by
-
Carriers of mitochondrial DNA macrohaplogroup L3 basal lineages migrated back to Africa from Asia around 70,000 years ago.BMC Evol Biol. 2018 Jun 19;18(1):98. doi: 10.1186/s12862-018-1211-4. BMC Evol Biol. 2018. PMID: 29921229 Free PMC article.
-
Biological Sexing of a 4000-Year-Old Egyptian Mummy Head to Assess the Potential of Nuclear DNA Recovery from the Most Damaged and Limited Forensic Specimens.Genes (Basel). 2018 Mar 1;9(3):135. doi: 10.3390/genes9030135. Genes (Basel). 2018. PMID: 29494531 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
