Reliable protein production in a Pseudomonas fluorescens expression system
- PMID: 21968453
- DOI: 10.1016/j.pep.2011.09.010
Reliable protein production in a Pseudomonas fluorescens expression system
Erratum in
- Protein Expr Purif. 2012 Oct;85(2):251
Abstract
A bottleneck to product development can be reliable expression of active target protein. A wide array of recombinant proteins in development, including an ever growing number of non-natural proteins, is being expressed in a variety of expression systems. A Pseudomonas fluorescens expression platform has been developed specifically for recombinant protein production. The development of an integrated molecular toolbox of expression elements and host strains, along with automation of strain screening is described. Examples of strain screening and scale-up experiments show rapid development of expression strains producing a wide variety of proteins in a soluble active form.
Copyright © 2011 Elsevier Inc. All rights reserved.
Similar articles
-
Soluble periplasmic production of human granulocyte colony-stimulating factor (G-CSF) in Pseudomonas fluorescens.Protein Expr Purif. 2011 Jul;78(1):69-77. doi: 10.1016/j.pep.2011.03.002. Epub 2011 Mar 17. Protein Expr Purif. 2011. PMID: 21396452
-
Cell-free protein synthesis using cell extract of Pseudomonas fluorescens and CspA promoter.Biochem Biophys Res Commun. 2004 Jun 25;319(2):671-6. doi: 10.1016/j.bbrc.2004.05.034. Biochem Biophys Res Commun. 2004. PMID: 15178458
-
Selection and characterization of a promoter for expression of single-copy recombinant genes in Gram-positive bacteria.BMC Biotechnol. 2005 Jan 14;5:3. doi: 10.1186/1472-6750-5-3. BMC Biotechnol. 2005. PMID: 15651989 Free PMC article.
-
[Recombinant protein production in Escherichia coli].Postepy Biochem. 2006;52(4):448-56. Postepy Biochem. 2006. PMID: 17536514 Review. Polish.
-
Tuning different expression parameters to achieve soluble recombinant proteins in E. coli: advantages of high-throughput screening.Biotechnol J. 2011 Jun;6(6):715-30. doi: 10.1002/biot.201100025. Epub 2011 May 12. Biotechnol J. 2011. PMID: 21567962 Review.
Cited by
-
Recombinant Pseudomonas growing on non-natural fluorinated substrates shows stress but overall tolerance to cytoplasmically released fluoride anion.mBio. 2024 Jan 16;15(1):e0278523. doi: 10.1128/mbio.02785-23. Epub 2023 Dec 8. mBio. 2024. PMID: 38063407 Free PMC article.
-
High-throughput process development from gene cloning to protein production.Microb Cell Fact. 2023 Sep 15;22(1):182. doi: 10.1186/s12934-023-02184-1. Microb Cell Fact. 2023. PMID: 37715258 Free PMC article. Review.
-
Recombinant vaccines in 2022: a perspective from the cell factory.Microb Cell Fact. 2022 Oct 5;21(1):203. doi: 10.1186/s12934-022-01929-8. Microb Cell Fact. 2022. PMID: 36199085 Free PMC article. Review.
-
Neuroinductive properties of mGDNF depend on the producer, E. Coli or human cells.PLoS One. 2021 Oct 11;16(10):e0258289. doi: 10.1371/journal.pone.0258289. eCollection 2021. PLoS One. 2021. PMID: 34634077 Free PMC article.
-
Platforms for Production of Protein-Based Vaccines: From Classical to Next-Generation Strategies.Biomolecules. 2021 Jul 21;11(8):1072. doi: 10.3390/biom11081072. Biomolecules. 2021. PMID: 34439738 Free PMC article. Review.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
