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Link to original content: https://pubmed.ncbi.nlm.nih.gov/18252098/
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. 2007 Sep;13(9):1295-300.
doi: 10.3201/eid1309.070491.

Detection of group 1 coronaviruses in bats in North America

Samuel R Dominguez  1 , Thomas J O'Shea, Lauren M Oko, Kathryn V Holmes
Affiliations

Detection of group 1 coronaviruses in bats in North America

Samuel R Dominguez et al. Emerg Infect Dis. 2007 Sep.

Abstract

The epidemic of severe acute respiratory syndrome (SARS) was caused by a newly emerged coronavirus (SARS-CoV). Bats of several species in southern People's Republic of China harbor SARS-like CoVs and may be reservoir hosts for them. To determine whether bats in North America also harbor coronaviruses, we used reverse transcription-PCR to detect coronavirus RNA in bats. We found coronavirus RNA in 6 of 28 fecal specimens from bats of 2 of 7 species tested. The prevalence of viral RNA shedding was high: 17% in Eptesicus fuscus and 50% in Myotis occultus. Sequence analysis of a 440-bp amplicon in gene 1b showed that these Rocky Mountain bat coronaviruses formed 3 clusters in phylogenetic group 1 that were distinct from group 1 coronaviruses of Asian bats. Because of the potential for bat coronaviruses to cause disease in humans and animals, further surveillance and characterization of bat coronaviruses in North America are needed.

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Figures

Figure 1
Figure 1
Nucleotide sequence alignment of amplicons from a 440-nt region of gene 1b of Rocky Mountain bat coronaviruses (RM-Bt-CoVs) compared with group 1 coronaviruses of Asian bats (BtCoVs) and human coronavirus 229E. Identical residues are shaded in blue and similar residues are shaded in yellow. Hyphens indicate positions where sequences are not available.
Figure 2
Figure 2
Phylogenetic relationships based on a 440-nt sequence in a conserved region of gene 1b of Rocky Mountain bat coronaviruses (RM-Bt-CoVs) (shown in boldface), group 1 coronaviruses of Asian bats (BtCoVs), and human coronaviruses 229E and NL63. Porcine respiratory and reproductive syndrome virus (PRRSV) was used as the outgroup to root the tree. Scale bar at the lower left indicates 0.1 nucleotide substitutions per site.
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