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Link to original content: https://pubmed.ncbi.nlm.nih.gov/17032622/
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. 2006 Nov;163(11):1203-6.
doi: 10.1016/j.jplph.2005.08.017. Epub 2005 Nov 7.

Proteins induced by cadmium in soybean cells

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Proteins induced by cadmium in soybean cells

Robert Sobkowiak et al. J Plant Physiol. 2006 Nov.

Abstract

The cadmium (Cd)-induced changes in protein pattern and identification of metal-stimulated polypeptides were analyzed in soybean cell suspension culture. The cell cultures were treated with various concentrations of Cd(2+) (3-10microM) for 24, 48 and 72h. The synthesis of [(35)S]-labeled proteins and their accumulation were analyzed by SDS-PAGE, whereas the identification of selected protein bands was performed by mass spectrometry. It is shown that Cd induced the appearance of the following proteins in soybean cells: superoxide dismutase, histone H2B, chalcone synthase and glutathione transferase.

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