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Link to original content: https://pubmed.ncbi.nlm.nih.gov/16000473/
Rapid detection of human metapneumovirus strains in nasopharyngeal aspirates and shell vial cultures by monoclonal antibodies - PubMed Skip to main page content
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. 2005 Jul;43(7):3443-6.
doi: 10.1128/JCM.43.7.3443-3446.2005.

Rapid detection of human metapneumovirus strains in nasopharyngeal aspirates and shell vial cultures by monoclonal antibodies

Elena Percivalle  1 , Antonella Sarasini, Livia Visai, M Grazia Revello, Giuseppe Gerna
Affiliations

Rapid detection of human metapneumovirus strains in nasopharyngeal aspirates and shell vial cultures by monoclonal antibodies

Elena Percivalle et al. J Clin Microbiol. 2005 Jul.

Abstract

Monoclonal antibodies to human metapneumovirus (hMPV) were developed for direct fluorescent antibody (DFA) staining of nasopharyngeal aspirates from 40 infants with respiratory infections, as well as for hMPV identification in shell vial cultures. With reference to reverse transcription-PCR, DFA staining showed sensitivity, specificity, and positive and negative predictive values of 73.9%, 94.1%, 94.4%, and 72.7%, respectively. Monoclonal antibodies are useful for direct hMPV detection.

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Figures

FIG. 1.
FIG. 1.
Patterns of IFA staining of MAbs to hMPV on hMPV-infected LLC-MK2 cell cultures. (A-B) granular pattern. (C-D) Filamentous pattern. (E-F) Foamy pattern.
FIG. 2.
FIG. 2.
(A-B) DFA staining of respiratory mucosal cells from two different NPAs. (C-D) hMPV isolation and identification in LLC-MK2 shell vial cell cultures by using MAbs 48 h after inoculation of two different NPA samples. Single infected cells are detected in panel C, while a plaque of infected cells with small syncytial formations is shown in panel D.
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