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Link to original content: https://pubmed.ncbi.nlm.nih.gov/15685193/
Microarray analysis shows that some microRNAs downregulate large numbers of target mRNAs - PubMed Skip to main page content
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. 2005 Feb 17;433(7027):769-73.
doi: 10.1038/nature03315. Epub 2005 Jan 30.

Microarray analysis shows that some microRNAs downregulate large numbers of target mRNAs

Lee P Lim  1 , Nelson C Lau, Philip Garrett-Engele, Andrew Grimson, Janell M Schelter, John Castle, David P Bartel, Peter S Linsley, Jason M Johnson
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Free article

Microarray analysis shows that some microRNAs downregulate large numbers of target mRNAs

Lee P Lim et al. Nature. .
Free article

Abstract

MicroRNAs (miRNAs) are a class of noncoding RNAs that post-transcriptionally regulate gene expression in plants and animals. To investigate the influence of miRNAs on transcript levels, we transfected miRNAs into human cells and used microarrays to examine changes in the messenger RNA profile. Here we show that delivering miR-124 causes the expression profile to shift towards that of brain, the organ in which miR-124 is preferentially expressed, whereas delivering miR-1 shifts the profile towards that of muscle, where miR-1 is preferentially expressed. In each case, about 100 messages were downregulated after 12 h. The 3' untranslated regions of these messages had a significant propensity to pair to the 5' region of the miRNA, as expected if many of these messages are the direct targets of the miRNAs. Our results suggest that metazoan miRNAs can reduce the levels of many of their target transcripts, not just the amount of protein deriving from these transcripts. Moreover, miR-1 and miR-124, and presumably other tissue-specific miRNAs, seem to downregulate a far greater number of targets than previously appreciated, thereby helping to define tissue-specific gene expression in humans.

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Comment in

  • Great expectations of small RNAs.
    Schuldt A. Schuldt A. Nat Rev Mol Cell Biol. 2010 Oct;11(10):676. doi: 10.1038/nrm2987. Nat Rev Mol Cell Biol. 2010. PMID: 20861869 No abstract available.

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