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Link to original content: https://pubmed.ncbi.nlm.nih.gov/15299701
X-ray diffraction analysis of crystals containing twofold symmetric nucleosome core particles - PubMed Skip to main page content
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. 1996 Mar 1;52(Pt 2):283-8.
doi: 10.1107/S0907444995009139.

X-ray diffraction analysis of crystals containing twofold symmetric nucleosome core particles

J M Harp  1 , E C Uberbacher, A E Roberson, E L Palmer, A Gewiess, G J Bunick
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X-ray diffraction analysis of crystals containing twofold symmetric nucleosome core particles

J M Harp et al. Acta Crystallogr D Biol Crystallogr. .

Abstract

Nucleosome core particles containing a DNA palindrome and purified chicken erythrocyte histone octamer have been reconstituted and crystallized. The dyad symmetry of the palindrome extends the dyad symmetry of the histone octamer to result in a twofold symmetric particle. This ensures that the structure determined by X-ray diffraction will yield a true representation of the DNA strand rather than the twofold averaged structure which would result from using a non-palindromic DNA sequence. The crystals provide isotropic diffraction to 3.2 A with observed reflections extending to d spacings of about 2.8 A using a rotating-anode Cu Kalpha X-ray source. Although the DNA palindrome is a factor contributing to the quality of the diffraction data, another significant factor is an improved preparative technique which enriches for correctly phased nucleosome core particles.

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