Abstract
PROGRESSION from G2 to M phase in eukaryotes requires activation of a protein kinase composed of p34cdc2/CDC28 associated with Gl-specific cyclins (reviewed in ref. 1). In some organisms the activation of the kinase at the G2/M boundary is due to dephosphorylation of a highly conserved tyrosine residue at posi-tion 15 (Y15) of the cdc2 protein2–6. Here we report that in the budding yeast Saccharomyces cervisiae, p34CDC28 also undergoes cell-cycle regulated dephosphorylation on an equivalent tyrosine residue (Y19). However, in contrast to previous observations in S. pombe6, Xenopus2,3 and mammalian cells4,5, dephosphorylation of Y19 is not required for the activation of the CDC28/cyclin kinase. Furthermore, mutation of this tyrosine residue does not affect dependence of mitosis on DNA synthesis nor does it abolish G2 arrest induced by DNA damage. Our data imply that regulated phosphorylation of this tyrosine residue is not the 'universal' means by which the onset of mitosis is determined. We propose that there are other unidentified controls that regulate entry into mitosis.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Pines, J. & Hunter, T. New Biol. 2, 389–401 (1990).
Dunphy, W. G. & Newport, J. W. Cell 58, 181–191 (1989).
Gautier, J., Matsukawa, T., Nurse, P. & Mailer, J. Nature 339, 626–629 (1989).
Draetta, G. & Beach, D. Cell 54, 17–26 (1988).
Morla, A. O., Draetta, G., Beach, D. & Wang, J. Y. J. Cell 58, 193–203 (1989).
Gould, K. L. & Nurse, P. Nature 342, 39–45 (1989).
Krek, W. & Nigg, E. A. EMBO J. 10, 305–316 (1991).
Krek, W. & Nigg, E. A. EMBO J. 10, 3331–3341 (1991).
Hartwell, L. & Weinert, T. Science 246, 629–634 (1989).
Weinert, T. & Hartwell, L. Science 241, 317–322 (1988).
Enoch, T. & Nurse, P. Cell 60, 665–673 (1990).
Nurse, P. Nature 344, 503–508 (1990).
Dunphy, W. G. & Kumagai A. Cell 67, 189–196 (1991).
Gautier, J., Solomon, M. J., Booher R. N., Bazan, J. F. & Kirschner M. Cell 67, 197–211 (1991).
Hartwell, L. J. Bact. 115, 966–974 (1973).
Dunphy, W. & Newport, J. Cell 55, 925–928 (1988).
Murray, A. W. & Kirschner M. W. Science 246, 614–621 (1989).
Rubin, G. M. Meth. Cell Biol. 12, 45–64 (1975).
Moll, T., Tebb, G., Surana, U., Robitsch, H. & Nasmyth, K. Cell 66, 743–758 (1991).
Cooper, J. A., Sefton, B. M. & Hunter, T. Meth. Enzym. 9, 387–402 (1983).
Gietz, R. D. & Sugino, A. Gene 74, 527–534 (1988).
Reed, S. I., Hadwiger, J. A. & Lorincz, A. T. Proc. natn. Acad. Sci. U.S.A. 82, 4055–4059 (1985).
Surana, U. et al. Cell 65, 145–161 (1991).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Amon, A., Surana, U., Muroff, I. et al. Regulation of p34CDC28 tyrosine phosphorylation is not required for entry into mitosis in S. cerevisiae. Nature 355, 368–371 (1992). https://doi.org/10.1038/355368a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/355368a0
This article is cited by
-
Methylglyoxal inhibits nuclear division through alterations in vacuolar morphology and accumulation of Atg18 on the vacuolar membrane in Saccharomyces cerevisiae
Scientific Reports (2020)
-
Budding yeast Wee1 distinguishes spindle pole bodies to guide their pattern of age-dependent segregation
Nature Cell Biology (2017)
-
The role of Cdc25 phosphatases in cell cycle checkpoints
Protoplasma (2000)
