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Link to original content: https://doi.org/10.1007/s11010-019-03655-z
Effects of 1,25-Dihydroxy vitamin D3 on TNF-α induced inflammation in human chondrocytes and SW1353 cells: a possible role for toll-like receptors | Molecular and Cellular Biochemistry Skip to main content
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Effects of 1,25-Dihydroxy vitamin D3 on TNF-α induced inflammation in human chondrocytes and SW1353 cells: a possible role for toll-like receptors

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Abstract

The purpose of this study is to evaluate anti-inflammatory and chondro-protective effects of 1,25(OH)2D3 in human chondrocytes and SW1353 cells via investigating expressions of MMPs, TIMPs, VDR, and intracellular signalling pathway mediators such as TLR-2 and -4. The HC and SW1353 cells were treated with 1,25(OH)2D3 at 10, 100, and 1000 nM concentrations in the absence/presence of TNF-α (20 ng/mL) for 48 h. The mRNA expressions of MMP-1, -2, -3, -9, and -13, TIMP-1 and -2, VDR, TLR-2 and -4 in HC and SW1353 cells were detected by qPCR after treatments. The cytotoxicity and cell proliferation analyses were assessed by LDH and WST-1 assay, respectively. Protein levels of MMPs, TIMPs, and VDR were analysed by immunocytochemistry and ELISA methods. TNF-α markedly increased cytotoxicity for 24, 48, 72 h (p < 0.05) and vitamin D treatment was shown to diminish the cytotoxic effect of TNF-α. Cell proliferations increased by Vitamin D in a dose-dependent manner. mRNA expressions of MMP-1, -2, -3, -9, and -13, TLR-2 and -4 genes decreased with 1,25(OH)2D3 treatment (p < 0.05). VDR, TIMP-1 and -2 levels elevated after TNF-α exposure compared with the control group in HC cells (p < 0.05). Protein expression levels were determined using Western blotting, ELISA and immunocytochemistry. 1,25(OH)2D3 via binding to VDR, reversed the effects of TNF-α by inhibiting TLR-2 and 4. Decreased levels of VDR, TIMP-1 and -2 after TNF-α treatment were elevated by 1,25(OH)2D3 proportional with increasing 1,25(OH)2D3 doses. 1,25(OH)2D3 and TNF-α co-treatment decreased MMP-1, -2, -3, -9, and -13 levels were after TNF-α exposure.

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Acknowledgements

This research was supported by a Grant from the Scientific Research Projects, Project Number 3510, Center in Ankara Yildirim Beyazit University 2017.

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Medicine, Ankara Yildirim Beyazit University, 06800, Ankara, Turkey

    Gamze Avcioglu, Betül Özbek Ipteç, Gulsen Yilmaz & Leyla Didem Kozaci

  2. Department of Medical Biology, Faculty of Medicine, Ankara Yildirim Beyazit University, Ankara, Turkey

    Ahmet Carhan

  3. Department of Histology and Embryology, Faculty of Medicine, Ankara Yildirim Beyazit University, Ankara, Turkey

    Gülben Akcan

  4. Department of Translational Medicine, Institute of Health Sciences, Ankara Yildirim Beyazit University, Ankara, Turkey

    Büsra Görgün, Kübra Fidan, Ahmet Carhan, Gulsen Yilmaz & Leyla Didem Kozaci

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  1. Gamze Avcioglu
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Avcioglu, G., Özbek Ipteç, B., Akcan, G. et al. Effects of 1,25-Dihydroxy vitamin D3 on TNF-α induced inflammation in human chondrocytes and SW1353 cells: a possible role for toll-like receptors. Mol Cell Biochem 464, 131–142 (2020). https://doi.org/10.1007/s11010-019-03655-z

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