iBet uBet web content aggregator. Adding the entire web to your favor.
iBet uBet web content aggregator. Adding the entire web to your favor.



Link to original content: https://doi.org/10.1007/s00253-003-1255-1
A novel catabolic activity of Pseudomonas veronii in biotransformation of pentachlorophenol | Applied Microbiology and Biotechnology Skip to main content

Advertisement

Log in

A novel catabolic activity of Pseudomonas veronii in biotransformation of pentachlorophenol

  • Original Paper
  • Published:
Applied Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

Pseudomonas veronii PH-05, a bacterial strain capable of transforming pentachlorophenol (PCP) to a metabolic intermediate, was isolated by selective enrichment of soil samples from a timber storage yard. Strain PH-05 was shown to be able to grow using PCP as the sole source of carbon and energy. GC-MS analysis showed that the metabolic intermediate was tetrachlorocatechol, which inhibited the growth of this strain. The formation of tetrachlorocatechol during biotransformation was monitored, and its inhibitory effect on growth of strain PH-05 was analyzed at a range of concentrations. The catabolic activity of the isolated strain differs from that of other PCP-degrading bacteria, which metabolize PCP through a chlorinated hydroquinone intermediate.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1.
Fig. 2.
Fig. 3.
Fig. 4.

Similar content being viewed by others

References

  • Anandarajah K, Kiefer PM, Donohoe BS Jr, Copley SD (2000) Recruitment of a double bond isomerase to serve as a reductive dehalogenase during biodegradation of pentachlorophenol. Biochemistry 39:5303–5311

    Article  CAS  PubMed  Google Scholar 

  • Apajalahti JHA, Salkinoja-Salonen MS (1986) Degradation of polychlorinated phenols by Rhodococcus chlorophenolicus, Appl Microbiol Biotechnol 25:62–67

    CAS  Google Scholar 

  • Apajalahti JHA, Salkinoja-Salonen MS (1987) Dechlorination and para-hydroxylation of polychlorinated phenols by Rhodococcus chlorophenolicus. J Bacteriol 169:675–681

    CAS  PubMed  Google Scholar 

  • Becaert V, Deschenes L, Samson R (2000) A simple method to evaluate the concentration of pentachlorophenol degraders in contaminated soils. FEMS Microbiol Lett 184:261–264

    Article  CAS  PubMed  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  CAS  PubMed  Google Scholar 

  • Chanama S, Crawford RL (1997) Mutational analysis of pcp A and its role in pentachlorophenol degradation by Sphingomonas (Flavobacterium) chlorophenolica ATCC 39723. Appl Environ Microbiol 63:4833–4838

    CAS  PubMed  Google Scholar 

  • Chaudri AM, Lawlor K, Mcgrath SP (2000) Pentachlorophenol utilization by indigenous soil microorganisms. Soil Biol Biochem 32:429–432

    Article  CAS  Google Scholar 

  • Chu JP, Kirsch EJ (1972) Metabolism of pentachlorophenol by an axenic bacterial culture. Appl Environ Microbiol 23:1033–1035

    CAS  Google Scholar 

  • Copley SD (2000) Evolution of a metabolic pathway for degradation of a toxic xenobiotic: the patchwork approach. Trends Biochem Sci 25:261–265

    CAS  PubMed  Google Scholar 

  • Häggblom MM, Nohynek LJ, Salkinoja-Salonen MS (1988) Degradation and O-methylation of chlorinated phenolic compounds by Rhodococcus and Mycobacterium strains. Appl Environ Microbiol 54:3043–3052

    PubMed  Google Scholar 

  • Hong HB, Chang YS, Choi SD, Nam IH, Lee YE (2001) Isolation and characterization of a cell-associated protein of Bacillus pumilus PH-01. Appl Microbiol Biotechnol 56:402–405

    Article  CAS  PubMed  Google Scholar 

  • Hong HB, Chang YS, Nam IH, Fortnagel P, Schmidt S (2002) Biotransformation of 2,7-dichloro- and 1,2,3,4-tetrachlorodibenzo-p-dioxin by Sphingomonas wittichii RW1. Appl Environ Microbiol 68:2584–2588

    Article  CAS  PubMed  Google Scholar 

  • Karns JS, Duttagupta S, Chakrabarty AM (1983a) Regulation of 2,4,5-trichlorophenoxyacetic acid and chlorophenol metabolism in Pseudomonas cepacia AC1100. Appl Environ Microbiol 46:1182–1186

    CAS  PubMed  Google Scholar 

  • Karns JS, Kilbane JJ, Duttagupta S, Chakrabarty AM (1983b) Metabolism of halophenols by 2,4,5-trichlorophenoxyacetic acid-degrading Pseudomonas cepacia. Appl Environ Microbiol 46:1176–1181

    CAS  PubMed  Google Scholar 

  • Lee JY, Xun L (1997) Purification and characterization of 2,6-dichloro-p-hydroquinone chlorohydrolase from Flavobacterium sp. Strain 39723. J Bacteriol 179:1521–1524

    CAS  PubMed  Google Scholar 

  • Leung KT, Campbell S, Gan Y, White DC, Lee H, Trevors JT (1999) The role of the Sphingomonas species UG30 pentachlorophenol-4-monooxygenase in p-nitrophenol degradation. FEMS Microbiol Lett 173:247–253

    Article  CAS  PubMed  Google Scholar 

  • McCarthy DL, Claude AA, Copley SD (1997) In vivo levels of chlorinated hydroquinones in a pentachlorophenol-degrading bacterium. Appl Environ Microbiol 63:1883–1888

    CAS  PubMed  Google Scholar 

  • Miyauchi K, Suh SK, Nagata Y, Takagi M (1998) Cloning and sequencing of a 2,5-dichlorohydroquinone reductive dehalogenase gene whose product is involved in degradation of γ-Hexachlorocyclohexane by Sphingomonas paucimobilis. J Bacteriol 180:1354–1359

    CAS  PubMed  Google Scholar 

  • Ohtsubo Y, Miyauchi K, Kanda K, Hatta T, Kiyohara H, Senda T, Nagata Y, Mitsui Y, Takagi M (1999) PcpA, which is involved in the degradation of pentachlorophenol in Sphingomonas chlorophenolica ATCC39723, is a novel type of ring-cleavage dioxygenase. FEBS Lett 459:395–398

    Article  CAS  PubMed  Google Scholar 

  • Saber DL, Crawford RL (1985) Isolation and characterization of Flavobacterium strains that degrade pentachlorophenol. Appl Environ Microbiol 50:1512–1518

    CAS  PubMed  Google Scholar 

  • Suzuki T (1977) Metabolism of pentachlorophenol by a soil microbe. J Environ Sci Health B 12:113–127

    CAS  PubMed  Google Scholar 

  • Wang H, Tiirola MA, Puhakka JA, Kulomaa MS, (2001) Production and characterization of the recombinant Sphingomonas chlorophenolica pentachlorophenol 4-monooxygenase. Biochem Biophys Res Commun 289:161–166

    Article  CAS  PubMed  Google Scholar 

  • Xun L, Topp E, Orser CS (1985) Confirmation of oxidative dehalogenation of pentachlorophenol by a Flavobacterium pentachlorophenol hydroxylase. J Bacteriol 174:5745–5747

    Google Scholar 

  • Xun L, Bohuslavek J, Cai M (1999) Characterization of 2,6-dichloro-p-hydroquinone 1,2-dioxygenase (PcpA) of Sphingomonas chlorophenolica ATCC 39723. Biochem Biophys Res Commun 266:322–325

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgement

This work was financially supported by Korea Research Foundation grant (KRF-2001-015-DP 0440).

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Y.-S. Chang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nam, IH., Chang, YS., Hong, HB. et al. A novel catabolic activity of Pseudomonas veronii in biotransformation of pentachlorophenol. Appl Microbiol Biotechnol 62, 284–290 (2003). https://doi.org/10.1007/s00253-003-1255-1

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00253-003-1255-1

Keywords

Navigation