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Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
IGF1	IBP1

Sequence Length (AA)	Molecular Weight (Da)
195	21841

Protein Name
Insulin-like growth factor I

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MGKISSLPTQ	LFKCCFCDFL	KVKMHTMSSS	HLFYLALCLL	TFTSSATAGP
60	70	80	90	100
ETLCGAELVD	ALQFVCGDRG	FYFNKPTGYG	SSSRRAPQTG	IVDECCFRSC
110	120	130	140	150
DLRRLEMYCA	PLKPAKSARS	VRAQRHTDMP	KTQKYQPPST	NKNTKSQRRK
160	170	180	190	195
GWPKTHPGGE	QKEGTEASLQ	IRGKKKEQRR	EIGSRNAECR	GKKGK

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3716 Collapse assay details

Data source: Panorama

Official Gene Symbol: Igf1
Peptide Sequence: GFYFNKPTGYGSSIR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 70
Peptide End: 84

CPTAC ID: non-CPTAC-3716
Peptide Molecular Mass: 1,692.8260
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y11 (2+)	18.8	9.1	6.8	22.4	11.7	8	29.2	14.8	10.5	15	15	15
y12 (2+)	14.8	6.6	6.1	14.9	6.2	5.4	21	9.1	8.1	15	15	15
y9 (1+)	9.5	6.7	5.7	10.8	10.6	6	14.4	12.5	8.3	15	15	15
b2 (1+)	9.4	6.6	7.6	19.5	9.2	7.3	21.6	11.3	10.5	15	15	15
y13 (3+)	13.8	5	3.4	15	5.4	5.2	20.4	7.4	6.2	15	15	15
sum	5	2.6	3.3	9.2	5.3	3.8	10.5	5.9	5	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-2677 Collapse assay details

Data source: Panorama

Official Gene Symbol: IGF1
Peptide Sequence: GFYFNKPTGYGSSSR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 70
Peptide End: 84

CPTAC ID: non-CPTAC-2677
Peptide Molecular Mass: 1,666.7740
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers