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Link to original content: https://antibodies.cancer.gov/detail/CPTC-SULT1E1-1
Catalog Number:
CPTC-SULT1E1-1
RRID:
AB_10805294
Target Antigen:
Sulfotransferase Family 1E, Estrogen-preferring, Member 1
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
05/28/2024
Antigen Recognition(s):
Recombinant Full-length, Endogenous
SOP:
Result: Positive
Imaging mass cytometry on colon cancer tissue core using CPTC-SULT1E1-1 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (liver, prostate, colon, pancreas, breast, lung, testis, endometrium, appendix, and kidney) and cancer tissues (breast and colon).
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org). ingle band corresponding to the predicted size in kDa (+/-20%). Analysis performed using a standard panel of samples. Antibody dilution: 1:500.
Result: Positive
Western Blot using CPTC-SULT1E1-1 as primary Ab against Ag 10897 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Catalog Number:
CPTC-SULT1E1-2
RRID:
AB_10804671
Target Antigen:
Sulfotransferase Family 1E, Estrogen-preferring, Member 1
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
11/26/2019
Antigen Recognition(s):
Recombinant Full-length, Endogenous
SOP:
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Result: Positive
Western Blot using CPTC-SULT1E1-2 as primary Ab against Ag 10897 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Catalog Number:
CPTC-SULT1E1-3
RRID:
AB_10804791
Target Antigen:
Sulfotransferase Family 1E, Estrogen-preferring, Member 1
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
11/26/2019
Antigen Recognition(s):
Recombinant Full-length, Endogenous
SOP:
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Result: Positive
Western Blot using CPTC-SULT1E1-3 as primary Ab against Ag 10897 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
NCI Identification Number:
10897
Antigen Name:
Sulfotransferase Family 1E, Estrogen-preferring, Member 1
CPTC Name:
CPTC-SULT1E1
Aliases:
EC 2.8.2.4; EST; EST-1; MGC34459; STE
Function:
Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters,drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure (number and length of exons) is similar among family members. This gene encodes a protein that transfers a sulfo moiety to and from estrone, which may control levels of estrogen receptors.
May control the level of the estrogen receptor by sulfurylating free estradiol. Maximally sulfates beta-estradiol and estrone at concentrations of 20 nM. Also sulfates dehydroepiandrosterone, pregnenolone, ethinylestradiol, equalenin, diethylstilbesterol and 1-naphthol, at significantly higher concentrations; however, cortisol, testosterone and dopamine are not sulfated.
Chromosomal Localization:
4q13.1
Expression System:
E. Coli
Accession Number:
BC027956.1
UniProt Accession Number:
P49888
DNA Source:
OB : MHS1010-7430106
Immunogen:
Recombinant Full Length Protein
Vector Name:
pMCSG7
Extinction Coefficient:
60975
Buffers:
PBS
Expressed Sequence:
SNAMNSELDYYEKFEEVHGILMYKDFVKYWDNVEAFQARPDDLVIATYPK
SGTTWVSEIVYMIYKEGDVEKCKEDVIFNRIPFLECRKENLMNGVKQLDE
MNSPRIVKTHLPPELLPASFWEKDCKIIYLCRNAKDVAVSFYYFFLMVAG
HPNPGSLPEFVEKFMQGQVPYGSWYKHVKSWWEKGKSPRVLFLFYEDLKE
DIRKEVIKLIHFLERKPSEELVDRIIHHTSFQEMKNNPSTNYTTLPDEIM
NQKLSPFMRKGITGDWKNHFTVALNEKFDKHYEQQMKESTLKFRTEI
Native Sequence:
MNSELDYYEKFEEVHGILMYKDFVKYWDNVEAFQARPDDLVIATYPKSGT
TWVSEIVYMIYKEGDVEKCKEDVIFNRIPFLECRKENLMNGVKQLDEMNS
PRIVKTHLPPELLPASFWEKDCKIIYLCRNAKDVAVSFYYFFLMVAGHPN
PGSLPEFVEKFMQGQVPYGSWYKHVKSWWEKGKSPRVLFLFYEDLKEDIR
KEVIKLIHFLERKPSEELVDRIIHHTSFQEMKNNPSTNYTTLPDEIMNQK
LSPFMRKGITGDWKNHFTVALNEKFDKHYEQQMKESTLKFRTEI
Calculated Isoelectric Point:
6.18
Molecular Weight:
35.09
Last Updated:
08/22/2020
PAGE of recombinant SULT1E1 (rAg 10897) (with molecular weight standards in lane 1)
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