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Link to original content: https://antibodies.cancer.gov/detail/CPTC-RAD52-1
Catalog Number:
CPTC-RAD52-1
RRID:
AB_2617327
Target Antigen:
RAD52 homolog (S. cerevisiae)
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
08/25/2021
Antigen Recognition(s):
Recombinant Full-length
SOP:
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Result: Positive
Western Blot using CPTC-RAD52-1 as primary Ab against PNMT (rAg 10468) in lane 2. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Catalog Number:
CPTC-RAD52-2
RRID:
AB_2617328
Target Antigen:
RAD52 homolog (S. cerevisiae)
Isotype:
IgG2a
Species:
Mouse Monoclonal Antibody
Last Updated:
08/25/2021
Antigen Recognition(s):
Recombinant Full-length
SOP:
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Result: Positive
Western Blot using CPTC-RAD52-2 as primary Ab against PNMT (rAg 10468) in lane 2. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Catalog Number:
CPTC-RAD52-3
RRID:
AB_2617329
Target Antigen:
RAD52 homolog (S. cerevisiae)
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
08/25/2021
Antigen Recognition(s):
Recombinant Full-length
SOP:
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Result: Positive
Western Blot using CPTC-RAD52-3 as primary Ab against PNMT (rAg 10468) in lane 2. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
NCI Identification Number:
10468
Antigen Name:
RAD52 homolog (S. cerevisiae)
CPTC Name:
CPTC-RAD52
Aliases:
RAD52 homolog (S. cerevisiae); DNA repair protein RAD52 homolog; recombination protein RAD52; rhabdomyosarcoma antigen MU-RMS-40.23
Function:
The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair.
Involved in double-stranded break repair. Plays a central role in genetic recombination and DNA repair by
promoting the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase
Chromosomal Localization:
12p13-p12.2
Expression System:
E. Coli
Accession Number:
U12134.1
UniProt Accession Number:
P43351
DNA Source:
DNASU - HsCD00022279
Immunogen:
Recombinant Full Length Protein
Vector Name:
pMCSG7
Extinction Coefficient:
40130
Buffers:
PBS without Ca++ and Mg++
Expressed Sequence:
SNAMSGTEEAILGGRDSHPAAGGGSVLCFGQCQYTAEEYQAIQKALRQRL
GPEYISSRMAGGGQKVCYIEGHRVINLANEMFGYNGWAHSITQQNVDFVD
LNNGKFYVGVCAFVRVQLKDGSYHEDVGYGVSEGLKSKALSLEKARKEAV
TDGLKRALRSFGNALGNCILDKDYLRSLNKLPRQLPLEVDLTKAKRQDLE
PSVEEARYNSCRPNMALGHPQLQQVTSPSRPSHAVIPADQDCSSRSLSSS
AVESEATHQRKLRQKQLQQQFRERMEKQQVRVSTPSAEKSEAAPPAPPVT
HSTPVTVSEPLLEKDFLAGVTQELIKTLEDNSEKWAVTPDAGDGVVKPSS
RADPAQTSDTLALNNQMVTQNRTPHSVCHQKPQAKSGSWDLQTYSADQRT
TGNWESHRKSQDMKKRKYDPS
Native Sequence:
MSGTEEAILGGRDSHPAAGGGSVLCFGQCQYTAEEYQAIQKALRQRLGPE
YISSRMAGGGQKVCYIEGHRVINLANEMFGYNGWAHSITQQNVDFVDLNN
GKFYVGVCAFVRVQLKDGSYHEDVGYGVSEGLKSKALSLEKARKEAVTDG
LKRALRSFGNALGNCILDKDYLRSLNKLPRQLPLEVDLTKAKRQDLEPSV
EEARYNSCRPNMALGHPQLQQVTSPSRPSHAVIPADQDCSSRSLSSSAVE
SEATHQRKLRQKQLQQQFRERMEKQQVRVSTPSAEKSEAAPPAPPVTHST
PVTVSEPLLEKDFLAGVTQELIKTLEDNSEKWAVTPDAGDGVVKPSSRAD
PAQTSDTLALNNQMVTQNRTPHSVCHQKPQAKSGSWDLQTYSADQRTTGN
WESHRKSQDMKKRKYDPS
Calculated Isoelectric Point:
8.49
Molecular Weight:
46441
Last Updated:
10/27/2011
PAGE of Ag 10468(with molecular weight standards in lane 1)
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