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Link to original content: http://www.ncbi.nlm.nih.gov/pubmed/8049364
Modification of carbonic anhydrase activity by antisense and over-expression constructs in transgenic tobacco - PubMed Skip to main page content
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. 1994 Jun;25(3):377-85.
doi: 10.1007/BF00043867.

Modification of carbonic anhydrase activity by antisense and over-expression constructs in transgenic tobacco

N Majeau  1 , M A Arnoldo, J R Coleman
Affiliations

Modification of carbonic anhydrase activity by antisense and over-expression constructs in transgenic tobacco

N Majeau et al. Plant Mol Biol. 1994 Jun.

Abstract

The activity and location of carbonic anhydrase has been modified by transformation of tobacco with antisense and over-expression constructs. Antisense expression resulted in the inhibition of up to 99% of carbonic anhydrase activity but had no significant impact on net CO2 assimilation. Stomatal conductance and susceptibility to water stress appeared to increase in response to the decline in carbonic anhydrase activity. An over-expression construct designed to increase cytosolic carbonic anhydrase abundance resulted in a significant increase in net activity, a small increase in stomatal conductance but little impact on CO2 assimilation. Chloroplastic carbonic anhydrase activity was enhanced by the expression of an additional construct which targeted the polypeptide to the organelle. The increase in chloroplastic carbonic anhydrase appeared to be accompanied by a concomitant increase in Rubisco activity.

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