Detection of staphylococcal enterotoxin A (SEA) at picogram level by a capacitive immunosensor
- PMID: 21500070
- DOI: 10.1080/10934529.2011.562810
Detection of staphylococcal enterotoxin A (SEA) at picogram level by a capacitive immunosensor
Abstract
This work presents the use of a flow injection capacitive immunosensor to detect staphylococcal enterotoxin A (SEA). The study was based on the direct detection of a capacitance change due to the binding between SEA and anti-SEA immobilized on a gold electrode. The optimal regeneration solution, flow rate, sample volume and buffer conditions were studied. Under the optimum conditions, this label-free biosensor provided linearity between 1 × 10(-12) g L(-1) and 1 × 10(-8) g L(-1) of SEA and the limit of detection was 1 × 10(-12) g L(-1) which was much lower than the infectious dose (0.5 × 10(-6) - 1 × 10(-6) g L(-1)). Using the regeneration solution of, 15.0 mM glycine-HCl pH 2.20, to break the binding between SEA and the immobilized anti-SEA enabled the electrode to be reused up to 39 times. This technique was applied to analyze SEA in liquid and solid food samples. Any matrix effect can be eliminated by simple dilution. SEA contamination was found in three samples, iced tea with milk (28 ± 1 ng L(-1)), orange juice (113 ± 6 ng L(-1)) and fried chicken (1.1 ± 0.2 ng g(-1)); however, the concentrations were much lower than the infectious dose. The proposed method would be useful for rapid screening of SEA in various matrices.
Similar articles
-
A novel electrochemical immunosensor based on magnetosomes for detection of staphylococcal enterotoxin B in milk.Talanta. 2013 Mar 15;106:360-6. doi: 10.1016/j.talanta.2012.12.053. Epub 2013 Jan 16. Talanta. 2013. PMID: 23598138
-
Piezoelectric immunosensor for direct and rapid detection of staphylococcal enterotoxin A (SEA) at the ng level.Biosens Bioelectron. 2011 Nov 15;29(1):140-4. doi: 10.1016/j.bios.2011.08.007. Epub 2011 Aug 10. Biosens Bioelectron. 2011. PMID: 21872461
-
Gold nanoparticle-based localized surface plasmon immunosensor for staphylococcal enterotoxin A (SEA) detection.Anal Bioanal Chem. 2017 Oct;409(26):6227-6234. doi: 10.1007/s00216-017-0563-8. Epub 2017 Aug 16. Anal Bioanal Chem. 2017. PMID: 28815272
-
High sensitivity chemiluminescence enzyme immunoassay for detecting staphylococcal enterotoxin A in multi-matrices.Anal Chim Acta. 2013 Sep 24;796:14-9. doi: 10.1016/j.aca.2013.07.044. Epub 2013 Jul 25. Anal Chim Acta. 2013. PMID: 24016577
-
[Temperature dependence of staphylococcal enterotoxin A production by Staphylococcus aureus].Nihon Rinsho. 2012 Aug;70(8):1323-8. Nihon Rinsho. 2012. PMID: 22894066 Review. Japanese.
Cited by
-
Immuno-SPR biosensor for the detection of Brucella abortus.Sci Rep. 2023 Dec 21;13(1):22832. doi: 10.1038/s41598-023-50344-5. Sci Rep. 2023. PMID: 38129569 Free PMC article.
-
Asymmetric Mach-Zehnder Interferometer Based Biosensors for Aflatoxin M1 Detection.Biosensors (Basel). 2016 Jan 6;6(1):1. doi: 10.3390/bios6010001. Biosensors (Basel). 2016. PMID: 26751486 Free PMC article.
-
Monoclonal antibody-based sandwich ELISA for the detection of staphylococcal enterotoxin A.Int J Environ Res Public Health. 2013 Apr 19;10(4):1598-608. doi: 10.3390/ijerph10041598. Int J Environ Res Public Health. 2013. PMID: 23603865 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical