Abstract
BACKGROUND—It has been suggested that eating green leafy vegetables, which are rich in lutein and zeaxanthin, may decrease the risk for age related macular degeneration. The goal of this study was to analyse various fruits and vegetables to establish which ones contain lutein and/or zeaxanthin and can serve as possible dietary supplements for these carotenoids. METHODS—Homogenates of 33 fruits and vegetables, two fruit juices, and egg yolk were used for extraction of the carotenoids with hexane. Measurement of the different carotenoids and their isomers was carried out by high performance liquid chromatography using a single column with an isocratic run, and a diode array detector. RESULTS—Egg yolk and maize (corn) contained the highest mole percentage (% of total) of lutein and zeaxanthin (more than 85% of the total carotenoids). Maize was the vegetable with the highest quantity of lutein (60% of total) and orange pepper was the vegetable with the highest amount of zeaxanthin (37% of total). Substantial amounts of lutein and zeaxanthin (30-50%) were also present in kiwi fruit, grapes, spinach, orange juice, zucchini (or vegetable marrow), and different kinds of squash. The results show that there are fruits and vegetables of various colours with a relatively high content of lutein and zeaxanthin. CONCLUSIONS—Most of the dark green leafy vegetables, previously recommended for a higher intake of lutein and zeaxanthin, have 15-47% of lutein, but a very low content (0-3%) of zeaxanthin. Our study shows that fruits and vegetables of various colours can be consumed to increase dietary intake of lutein and zeaxanthin. Keywords: age related macular degeneration; carotenoids; dietary intake; macular pigment
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- Bone R. A., Landrum J. T., Fernandez L., Tarsis S. L. Analysis of the macular pigment by HPLC: retinal distribution and age study. Invest Ophthalmol Vis Sci. 1988 Jun;29(6):843–849. [PubMed] [Google Scholar]
- Clevidence B. A., Bieri J. G. Association of carotenoids with human plasma lipoproteins. Methods Enzymol. 1993;214:33–46. doi: 10.1016/0076-6879(93)14051-j. [DOI] [PubMed] [Google Scholar]
- Forman M. R., Lanza E., Yong L. C., Holden J. M., Graubard B. I., Beecher G. R., Meltiz M., Brown E. D., Smith J. C. The correlation between two dietary assessments of carotenoid intake and plasma carotenoid concentrations: application of a carotenoid food-composition database. Am J Clin Nutr. 1993 Oct;58(4):519–524. doi: 10.1093/ajcn/58.4.519. [DOI] [PubMed] [Google Scholar]
- Gerster H. Review: antioxidant protection of the ageing macula. Age Ageing. 1991 Jan;20(1):60–69. doi: 10.1093/ageing/20.1.60. [DOI] [PubMed] [Google Scholar]
- Ginsberg H. N., Karmally W., Siddiqui M., Holleran S., Tall A. R., Rumsey S. C., Deckelbaum R. J., Blaner W. S., Ramakrishnan R. A dose-response study of the effects of dietary cholesterol on fasting and postprandial lipid and lipoprotein metabolism in healthy young men. Arterioscler Thromb. 1994 Apr;14(4):576–586. doi: 10.1161/01.atv.14.4.576. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hammond B. R., Jr, Fuld K., Curran-Celentano J. Macular pigment density in monozygotic twins. Invest Ophthalmol Vis Sci. 1995 Nov;36(12):2531–2541. [PubMed] [Google Scholar]
- Hammond B. R., Jr, Fuld K., Snodderly D. M. Iris color and macular pigment optical density. Exp Eye Res. 1996 Mar;62(3):293–297. doi: 10.1006/exer.1996.0035. [DOI] [PubMed] [Google Scholar]
- Hammond B. R., Jr, Johnson E. J., Russell R. M., Krinsky N. I., Yeum K. J., Edwards R. B., Snodderly D. M. Dietary modification of human macular pigment density. Invest Ophthalmol Vis Sci. 1997 Aug;38(9):1795–1801. [PubMed] [Google Scholar]
- Handelman G. J., Dratz E. A., Reay C. C., van Kuijk J. G. Carotenoids in the human macula and whole retina. Invest Ophthalmol Vis Sci. 1988 Jun;29(6):850–855. [PubMed] [Google Scholar]
- Jacques P. F., Chylack L. T., Jr Epidemiologic evidence of a role for the antioxidant vitamins and carotenoids in cataract prevention. Am J Clin Nutr. 1991 Jan;53(1 Suppl):352S–355S. doi: 10.1093/ajcn/53.1.352S. [DOI] [PubMed] [Google Scholar]
- Khachik F., Bernstein P. S., Garland D. L. Identification of lutein and zeaxanthin oxidation products in human and monkey retinas. Invest Ophthalmol Vis Sci. 1997 Aug;38(9):1802–1811. [PubMed] [Google Scholar]
- Krinsky N. I. Actions of carotenoids in biological systems. Annu Rev Nutr. 1993;13:561–587. doi: 10.1146/annurev.nu.13.070193.003021. [DOI] [PubMed] [Google Scholar]
- Landrum J. T., Bone R. A., Kilburn M. D. The macular pigment: a possible role in protection from age-related macular degeneration. Adv Pharmacol. 1997;38:537–556. doi: 10.1016/s1054-3589(08)60998-9. [DOI] [PubMed] [Google Scholar]
- Malinow M. R., Feeney-Burns L., Peterson L. H., Klein M. L., Neuringer M. Diet-related macular anomalies in monkeys. Invest Ophthalmol Vis Sci. 1980 Aug;19(8):857–863. [PubMed] [Google Scholar]
- Mangels A. R., Holden J. M., Beecher G. R., Forman M. R., Lanza E. Carotenoid content of fruits and vegetables: an evaluation of analytic data. J Am Diet Assoc. 1993 Mar;93(3):284–296. doi: 10.1016/0002-8223(93)91553-3. [DOI] [PubMed] [Google Scholar]
- Mares-Perlman J. A., Brady W. E., Klein R., Klein B. E., Bowen P., Stacewicz-Sapuntzakis M., Palta M. Serum antioxidants and age-related macular degeneration in a population-based case-control study. Arch Ophthalmol. 1995 Dec;113(12):1518–1523. doi: 10.1001/archopht.1995.01100120048007. [DOI] [PubMed] [Google Scholar]
- Micozzi M. S. Evaluation of carotenoid intake. Methods Enzymol. 1993;214:17–21. doi: 10.1016/0076-6879(93)14049-o. [DOI] [PubMed] [Google Scholar]
- Schalch W., Weber P. Vitamins and carotenoids--a promising approach to reducing the risk of coronary heart disease, cancer and eye diseases. Adv Exp Med Biol. 1994;366:335–350. doi: 10.1007/978-1-4615-1833-4_24. [DOI] [PubMed] [Google Scholar]
- Schnohr P., Thomsen O. O., Riis Hansen P., Boberg-Ans G., Lawaetz H., Weeke T. Egg consumption and high-density-lipoprotein cholesterol. J Intern Med. 1994 Mar;235(3):249–251. doi: 10.1111/j.1365-2796.1994.tb01068.x. [DOI] [PubMed] [Google Scholar]
- Scita G., Aponte G. W., Wolf G. Uptake and cleavage of beta-carotene by cultures of rat small intestinal cells and human lung fibroblasts. Methods Enzymol. 1993;214:21–32. doi: 10.1016/0076-6879(93)14050-s. [DOI] [PubMed] [Google Scholar]
- Seddon J. M., Ajani U. A., Sperduto R. D., Hiller R., Blair N., Burton T. C., Farber M. D., Gragoudas E. S., Haller J., Miller D. T. Dietary carotenoids, vitamins A, C, and E, and advanced age-related macular degeneration. Eye Disease Case-Control Study Group. JAMA. 1994 Nov 9;272(18):1413–1420. [PubMed] [Google Scholar]
- Snodderly D. M. Evidence for protection against age-related macular degeneration by carotenoids and antioxidant vitamins. Am J Clin Nutr. 1995 Dec;62(6 Suppl):1448S–1461S. doi: 10.1093/ajcn/62.6.1448S. [DOI] [PubMed] [Google Scholar]
- Snodderly D. M., Handelman G. J., Adler A. J. Distribution of individual macular pigment carotenoids in central retina of macaque and squirrel monkeys. Invest Ophthalmol Vis Sci. 1991 Feb;32(2):268–279. [PubMed] [Google Scholar]
- Sommerburg O., Zang L. Y., van Kuijk F. J. Simultaneous detection of carotenoids and vitamin E in human plasma. J Chromatogr B Biomed Sci Appl. 1997 Aug 1;695(2):209–215. doi: 10.1016/s0378-4347(97)00036-4. [DOI] [PubMed] [Google Scholar]
- West S., Vitale S., Hallfrisch J., Muñoz B., Muller D., Bressler S., Bressler N. M. Are antioxidants or supplements protective for age-related macular degeneration? Arch Ophthalmol. 1994 Feb;112(2):222–227. doi: 10.1001/archopht.1994.01090140098031. [DOI] [PubMed] [Google Scholar]
- Yeum K. J., Taylor A., Tang G., Russell R. M. Measurement of carotenoids, retinoids, and tocopherols in human lenses. Invest Ophthalmol Vis Sci. 1995 Dec;36(13):2756–2761. [PubMed] [Google Scholar]
- Young R. W. Solar radiation and age-related macular degeneration. Surv Ophthalmol. 1988 Jan-Feb;32(4):252–269. doi: 10.1016/0039-6257(88)90174-9. [DOI] [PubMed] [Google Scholar]