Yeast Gateway Kit
(Kit # 1000000011 )

Original Publication

A suite of Gateway cloning vectors for high-throughput genetic analysis in Saccharomyces cerevisiae . Alberti S, Gitler AD, and Lindquist S. Yeast 2007 Oct;24(10):913-9. PubMed PMID 17583893.

Description

The Susan Lindquist laboratory of the Howard Hughes Medical Institute and the Whitehead Institute for Biomedical Research has deposited a set of Gateway-compatible destination vectors for high-throughput genetic analysis in S. cerevisiae . This kit contains 283 yeast Gateway destination vectors based on the widely used pRS series of yeast shuttle vectors and contain the two commonly used GPD and GAL1 promoter expression systems that enable expression of ORFs either constitutively or under galactose-inducible conditions. Additionally, proteins of interest can be fused to a choice of frequently used N or C terminal tags, such as EGFP, ECFP, EYFP, Cerulean, DsRed, HA, or TAP.

Please note that Addgene has not sequence verified the origin of replication in most of these plasmids. Scientists requesting this kit are strongly recommended to sequence the origin (particularly plasmids containing the CEN origin) as well as any features not confirmed by Addgene's quality control results, which can be located on each individual plasmid webpage. See Contents of Kit tab section for links to plasmid pages.

Known Issues: Plasmids in wells E8 of Plate #1 E8, E11 and F11 of Plate #2 and well E5 of Plate #3 are duplicates of other plasmids in the kit and should not be used. Plasmids in wells A7, B7, C7, D7, G7 and H7 of Plate #2 are missing the CYC1 terminator. The plasmid in well A8 of Plate #3 is missing a portion of the Gal1 promoter. It is unknown how these deletions will affect gene expression.

Kit Documentation

Resources

Download Plasmid Sequences in Tab Delimited Format:

Please note that the full plasmid sequences are provided as a reference only, and may not match the exact sequence of the plasmid. Please see Addgene's sequencing results for the most accurate information.

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:
"The S. cerevisiae Advanced Gateway Destination Vectors were a gift from Susan Lindquist (Addgene kit #1000000011)"

For your Reference section:

A suite of Gateway cloning vectors for high-throughput genetic analysis in Saccharomyces cerevisiae . Alberti S, Gitler AD, and Lindquist S. Yeast 2007 Oct;24(10):913-9. PubMed PMID 17583893.