MoBiFC - a modular bimolecular fluorescence complementation toolkit for analyzing protein-protein interactions in plants
(Kit #
1000000222
)
Depositing Lab: Benjamin Field
The modular bimolecular fluorescence complementation (MoBiFC) toolkit allows for the analysis of protein-protein interactions in plants.
This kit will be sent as bacterial glycerol stocks in 96-well plate format.
Original Publication
MoBiFC: development of a modular bimolecular fluorescence complementation toolkit for the analysis of chloroplast protein-protein interactions. Velay F, Soula M, Mehrez M, Belbachir C, D'Alessandro S, Laloi C, Crete P, Field B. Plant Methods. 2022 May 26;18(1):69. doi: 10.1186/s13007-022-00902-1. PubMed (Link opens in a new window) Article (Link opens in a new window).
Description
The MoBiFC toolkit is a collection of Golden Gate/MoClo compatible vectors for assembling single-TDNA bimolecular fluorescence complementation experiments in plants. It includes positive and negative interaction controls for chloroplast BiFC experiments, as well as TurboID modules for proximity labelling experiments.
(A) Outline of a multigenic MoBiFC construct for testing an interaction between two proteins of interest (POIs) by BiFC. The example is shown with chloroplast target peptides (green) and C-terminal YFP fragment fusions (yellow; n, N-ter fragment; c, C-ter fragment). OEP7-TRQ is a reference fluorescent protein for transformed cells. (B) Images displaying BiFC assays between two protein pairs in N. benthamiana mesophyll cells. The HSP21-cYFP HSP21-nYFP interaction allows the reconstitution of the YFP fragments into functional YFP, while the negative control using mCHERRY-nYFP does not. Scale, 10 µm; histogram levels are shown at the bottom of each channel.
Figure from Velay et al. Plant Methods. 2022 May 26;18(1):69. doi: 10.1186/s13007-022-00902-1. PubMed (Link opens in a new window) Article (Link opens in a new window). Reprinted under the terms of the Creative Commons Attribution License.
Additional Documentation
A list of modules (Additional File 2) and a cloning protocol (Additional File 3) can be found in the associated publication below.
How to Cite this Kit
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.
For your Materials and Methods section:
“MoBiFC was a gift from Benjamin Field (Addgene kit #1000000222).”
For your Reference section:
MoBiFC: development of a modular bimolecular fluorescence complementation toolkit for the analysis of chloroplast protein-protein interactions. Velay F, Soula M, Mehrez M, Belbachir C, D'Alessandro S, Laloi C, Crete P, Field B. Plant Methods. 2022 May 26;18(1):69. doi: 10.1186/s13007-022-00902-1. PubMed (Link opens in a new window) Article (Link opens in a new window).
MoBiFC - #1000000222
- Resistance Color Key
Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.
- Inventory
Searchable and sortable table of all plasmids in kit. The Well column lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.
- Kit Plate Map
96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.
Resistance Color Key
| Spectinomycin | |
| Kanamycin | |
| Ampicillin |
Inventory
| Well | Plasmid | Resistance |
|---|---|---|
| A / 1 | pLBC0010 | Spectinomycin |
| A / 2 | pLBC0011 | Spectinomycin |
| A / 3 | pLBC0020 | Spectinomycin |
| A / 4 | pLBC0030 | Spectinomycin |
| A / 5 | pLBC0031 | Spectinomycin |
| A / 6 | pLBC0040 | Spectinomycin |
| A / 7 | pLBC0041 | Spectinomycin |
| A / 8 | pLBC0050 | Spectinomycin |
| A / 9 | pLBC0051 | Spectinomycin |
| A / 10 | pLBC0060 | Spectinomycin |
| A / 11 | pLBC0061 | Spectinomycin |
| A / 12 | pLBC0071 | Spectinomycin |
| B / 1 | pLBC0080 | Spectinomycin |
| B / 2 | pLBC0081 | Spectinomycin |
| B / 3 | pLBC0090 | Spectinomycin |
| B / 4 | pLBC0100 | Spectinomycin |
| B / 5 | pLBC0110 | Spectinomycin |
| B / 6 | pLBC0120 | Spectinomycin |
| B / 7 | pLBC0130 | Spectinomycin |
| B / 8 | pLBC0131 | Spectinomycin |
| B / 9 | pLBC0140 | Spectinomycin |
| B / 10 | pLBC0150 | Spectinomycin |
| B / 11 | pLBC0151 | Kanamycin |
| B / 12 | pLBT0010 | Ampicillin |
| C / 1 | pLBT0020 | Ampicillin |
| C / 2 | pLBT0030 | Ampicillin |
| C / 3 | pLBT0040 | Ampicillin |
| C / 4 | pLBT0050 | Ampicillin |
| C / 5 | pLBT0060 | Ampicillin |
| C / 6 | pLBT0070 | Ampicillin |
| C / 7 | pLBT0080 | Ampicillin |
| C / 8 | pLBT0090 | Ampicillin |
| C / 9 | pLBT0100 | Ampicillin |
| C / 10 | pLBT0110 | Ampicillin |
| C / 11 | pLBT0120 | Ampicillin |
| C / 12 | pLBT0130 | Ampicillin |
| D / 1 | pLBT0140 | Ampicillin |
| D / 2 | pLBT0150 | Ampicillin |
| D / 3 | pLBT0160 | Ampicillin |
| D / 4 | pLBT0170 | Ampicillin |
| D / 5 | pLBT0180 | Ampicillin |
| D / 6 | pLBT0190 | Ampicillin |
| D / 7 | pLBM0010 | Kanamycin |
| D / 8 | pLBM0020 | Kanamycin |
| D / 9 | pLBM0030 | Kanamycin |
| D / 10 | pLBM0040 | Kanamycin |
| D / 11 | pLBM0050 | Kanamycin |
| D / 12 | pLBM0060 | Kanamycin |
| E / 1 | pLBM0070 | Kanamycin |
| E / 2 | pLBM0090 | Kanamycin |