Mouse Metabolism Library
(Pooled Library #163966)
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Purpose
This lentiviral CRISPR library targets mouse metabolic genes with ~6 guide RNAs (sgRNAs) per gene.
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Vector Backbone
lentiCRISPR v2 (Plasmid #52961) - expresses Cas9
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Depositing Labs
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | ||
|---|---|---|---|---|---|---|
| Pooled Library | 163966 | Mouse Metabolism Library | 1 | $430 | Add to Cart | |
Library Details
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SpeciesMouse
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Genes targeted2,918
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gRNAs18,343
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Controls1,000 intergenic controls
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Lentiviral Generation3rd
Library Shipping
This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.
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Volume∼20 µL
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Concentration50 ng/µL
Resource Information
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Protocols
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Depositor Data
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Depositor software
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Terms and Licenses
Academic/Nonprofit TermsIndustry Terms- Not Available to Industry
Trademarks- Zeocin® is an InvivoGen trademark.
Depositor Comments
The depositing lab has shared a GitHub repository (Link opens in a new window) with code for screening analysis.
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Mouse Metabolism Library was a gift from Alec Kimmelman (Addgene #163966) -
For your References section:
Functional Genomics Identifies Metabolic Vulnerabilities in Pancreatic Cancer. Biancur DE, Kapner KS, Yamamoto K, Banh RS, Neggers JE, Sohn ASW, Wu W, Manguso RT, Brown A, Root DE, Aguirre AJ, Kimmelman AC. Cell Metab. 2020 Oct 28. pii: S1550-4131(20)30551-9. doi: 10.1016/j.cmet.2020.10.0186. PubMed 33152323 (Link opens in a new window)