Dow Lab Base Editing Sensor Libraries
(Pooled Library #179217, #179218, #179219)
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Purpose
The Mouse Base Editing Sensor (MBES) and Human Base Editing Sensor (HBES) libraries are made up of sgRNAs that target frequently occurring cancer-associated mutations identified in the MSK IMPACT dataset that can be modeled with cytosine base editing. The ALL-PAM sensor (AP) library is made up of 10 human and mouse sgRNAs, where for each sgRNA the tethered target site has 1 of 64 possible 3-bp PAM sequences. Notably these "sensor libraries" also contain the cognate genomic target site within the same expression cassette for quantification of base editing efficiency.
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Vector Backbone
pLenti-U6-tdTomato-P2A-BlasR (LRT2B) (Plasmid #110854) - does not express base editor
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Depositing Labs
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | ||
|---|---|---|---|---|---|---|
| Pooled Library | 179217 | Mouse Base Editing Sensor Library† | 1 | $380 | Add to Cart | |
| Pooled Library | 179218 | Human Base Editing Sensor Library† | 1 | $380 | Add to Cart | |
| Pooled Library | 179219 | ALL-PAM sensor Library† | 1 | $380 | Add to Cart | |
† A base editor plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with any cytosine base editor plasmid, such as FNLS (high editing efficiency), F2X (expanded editing range), or FNLS-NG (PAM flexibility) or others.
Library Details
| Library | Species | Genes Targeted | No. gRNAs |
|---|---|---|---|
| MBES | Mouse | 462 | 4,686 |
| HBES | Human | 462 | 5,855 |
| All-PAM | Mouse & Human | 20 | 1,280 |
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Lentiviral Generation3rd
Library Shipping
This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.
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Volume∼20 µL
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Concentration50 ng/µL
Resource Information
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Protocols
- Library Amplification Protocol
(This protocol should be adjusted to use 50 ng of library DNA for transformation.)
- Library Amplification Protocol
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Depositor Data
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Terms and Licenses
Academic/Nonprofit TermsIndustry Terms- Not Available to Industry
Trademarks- Zeocin® is an InvivoGen trademark.
Depositor Comments
Explore the library targets and browse guide RNAs by species, gene, target mutation, and/or base editor at the BE-SCAN web application (Link opens in a new window). The base editor sensor libraries were generated using the AMINEsearch pipeline available on GitHub (Link opens in a new window).
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Mouse Base Editing Sensor Library was a gift from Lukas Dow (Addgene #179217)
Human Base Editing Sensor Library was a gift from Lukas Dow (Addgene #179218)
ALL-PAM Sensor Library was a gift from Lukas Dow (Addgene #179219) -
For your References section:
Base editing sensor libraries for high-throughput engineering and functional analysis of cancer-associated single nucleotide variants. Sánchez-Rivera FJ, Diaz BJ, Kastenhuber ER, Schmidt H, Katti A, Kennedy M, Tem V, Ho Y-J, Leibold J, Paffenholz SV, Barriga FM, Chu K, Goswami S, Wuest AN, Simon JM, Tsanov KM, Chakravarty D, Zhang H, Leslie CS, Lowe SW, Dow LE. Nat Biotechnol. 2022 Feb 14. doi: 10.1038/s41587-021-01172-3. PMID 35165384