Pooled Libraries

This lentiviral genome-scale CRISPR library targets all ~20,500 protein-coding genes in the human genome. The library is divided into 9 sublibraries defined by biological categories of genes, each with their own negative controls.

This lentiviral genome-scale CRISPR library targets all ~23,000 protein-coding genes in the mouse genome. The library is divided into 10 sublibraries defined by biological categories, and each of these is then split randomly by gene into two sublibraries (A and B).

This lentiviral CRISPR library induces knockouts of paralogous gene pairs. Plasmids express a pair of guide RNAs targeting either individual genes or a pair of paralogs.

This lentiviral CRISPR knockout library targets human epigenetic genes.

This lentiviral CRISPR library targets mouse metabolic genes and includes most metabolic enzymes and transporters.

These lentiviral CRISPR libraries target mouse lipid and sphingolipid metabolic genes and can be used to find lipid metabolic vulnerabilities in mouse cells.

Lentiviral CRISPR library targets human ubiquitation-related proteins and deubiquitinating enzymes.

Yeast CRISPR knockout libraries targeting transporter genes. Five sub-pools target different groups of transporters and a sixth library targets all 361 genes.

This lentiviral CRISPR library targets 3' splice sites with paired guide RNAs. It induces skipping of poison cassette exons and corresponding upstream constitutive exons. Directly compare loss of a constitutive coding exon with loss of a poison exon.

The mouse pKO library targets proximal poly(A) signal sequences in genes identified to be differentially polyadenylated in the mouse B16-F10 melanoma system.

CRISPR KO library targeting a comprehensive set of mouse genes with annotated function as chromatin regulators.

Lentiviral CRISPR knockout library targets human DNA damage response and DNA repair genes.

Lentiviral CRISPR knockout library targets mouse cell surface genes.

This AAV CRISPR knockout library targets mouse tumor suppressor genes (TSG). Each plasmid carries one TSG-targeting sgRNA in addition to one p53-targeting sgRNA. Because the backbone expresses both luciferase and Cre, the library can be used to interrogate tumorigenesis in vivo using a mouse line expressing Cre-dependent Cas9.

This lentiviral library is a CRISPR-Cpf1/Cas12a double knockout library. There are 26 genes targeted in the library, chosen from analysis of human metastasis cohorts and a prior mouse metastasis CRISPR screen. It can be used to interrogate the genetic interactions driving metastasis.

Designed using optimized metrics that are described in Doench et al. (2016), which combined improved on-target activity predictions (Rule Set 2), with an off-target score, the Cutting Frequency Determination (CFD); John Doench’s gRNA design blog post describes how gRNAs were designed for this library.

CRISPR knockout pooled library that targets mouse kinome genes

Designed using optimized metrics that are described in Doench et al. (2016), which combined improved on-target activity predictions (Rule Set 2), with an off-target score, the Cutting Frequency Determination (CFD); John Doench’s gRNA design blog post describes how gRNAs were designed for this library.

CRISPR knockout pooled library that targets kinome genes

Designed for assays with limited cell numbers, this can be used with John Doench’s Brunello human genome-wide library.

Designed for assays with limited cell numbers, this can be used with John Doench’s Brie mouse genome-wide library.

Lentiviral CRISPR knockout library targeting green monkey (Chlorocebus sabaeus) genome.

The human CRISPR knockout enAsCas12a sgRNA libraries were designed for assays with limited cell numbers.

The Inzolia genome-scale human library, with a five-fold reduction in the number of clones required, can be used for assaying genetic interactions.

Base editor sgRNAs target frequently occurring cancer-associated mutations that can be modeled with cytosine base editing.

Each gene is targeted by an AsCpf1(AsCas12a)-based array containing 3-4 guides concatenated in one vector.

CRISPR knockout pooled library targets human E-boxes genome-wide.

Lentiviral CRISPR knockout library targets ubiquitin ligases, deubiquitinases, and control genes.

CRISPR knockout pooled library targets a set of human interferon-stimulated genes (ISGs).

Human genome-wide knockout library designed with two sgRNAs per gene.

SgRNA library tiles complete KSHV genome for pooled screening.

Paired guide RNA library targets genes related to B-cell to macrophage transdifferentiation.

This CRISPR knockout library targets mouse metabolic genes.

This library can be used for genome-scale screening in the mouse liver.

CRISPR knockout pooled library targets a set of human transcription factors for LOF screening.

Human CRISPR Knockout Library targets ubiquitination and de-ubiquitination complex genes.

This pooled CRISPR library targets 1,594 primary human miRNA genes. The library contains 4-5 gRNAs per gene and 1,000 non-targeting control gRNAs for a total of 8,382 gRNAs.

This pooled library is designed for genomic targeting and screening in Drosophila cells. To design the gRNAs, the depositing laboratory computed all possible gRNA target sites within the shared exonic regions on either DNA strand of the Drosophila genome.

The CRISPR knockout library contains ~10,000 sgRNAs targeting 1,980 human druggable genes (5 sgRNAs per gene and 100 non-targeting control sgRNAs).

Delivered as two half-libraries h1 and h2. Designed using optimized metrics that are described in Xu et al. (2015), which (1) maximize on-target cleavage effects using the SSC algorithm developed by the Liu Lab ( Xu et al. Genome Research 2015), (2) minimize potential off-target effects, and (3) ensure the fidelity of their sequence with corresponding target loci and avoid regions with possible genomic variations.

Delivered as two half-libraries M1 and M2, the guide RNAs in this genome-wide CRISPR knockout library are designed to maximize on-target cleavage while minimizing off-target effects.

Delivered as two half-libraries Library A and Library B. The guide RNAs in these CRISPR knockout libraries target mouse genes implicated in tumor initiation, progression, and immune modulation.

Used for first apicomplexan parasite knockout screen.

The Advanced Catalogue of Epigenetic Regulators (ACER) library consists 748 traditional and newly predicted epigenetic regulators using epigenetic network analyses that integrated publicly available gene codependency (DepMap) and protein-protein interaction data (BioPlex).

CRISPR knockout library targets cancer-related genes.

Two pooled libraries express hybrid Cas9-Cas12a guide RNAs under a single promoter. Used to identify genetic interactions between paralogs and investigate alternative exon function.

Targets mouse protein-coding genes with 4-5 gRNAs/gene. Library includes 418 control gRNAs.

Library targets 182 immune evasion genes allow LOF screening against genes shown to increase sensistivity or resistance of cancer cells to CTRL-mediated toxicity.

Targets nearly all human protein-coding genes. Base library: gRNAs with no predicted off-target sites. Supplemental library: 6 additional gRNAs per gene.

The Toronto KnockOut Library v3 (TKOv3) is contains 70,948 gRNAs targeting 18,053 protein coding genes (4 gRNAs/gene) with 142 control gRNAs targeting EGFP, LacZ, and luciferase (71,090 total). It is available in both one- and two-plasmid systems.

The gRNAs are sequence-optimized based on the criteria described in the associated article, and the number of gRNAs per gene is decreased to 4 based on the finding that there are diminishing benefits to more gRNAs per gene beyond four.

CRISPR screen that is useful for the exploration of lipid biology and metabolism.

CRISPR knockout library targets human genes involved in cellular glycosylation.

Lentiviral CRISPR knockout library delivers random pairs of optimised gRNAs, reducing screens scale with similar performance to larger libraries.

Designed for long-term sgRNA delivery in Drosophila cell lines.

AAV CRISPR Library targets transcriptional regulators expressed in cardiomyocytes.

Genome-scale pan-cancer interrogation of lncRNA dependencies using CasRx.

Optimized for cleavage activity in order to maximize the likelihood of gene knockout. Split into three sub-libraries, each containing 10 gRNAs per gene.

Lentiviral CRISPR knockout library targets human genes required for mTORC1 activation and inhibition.

Optimized for cleavage activity in order to maximize the likelihood of gene knockout. Split into three sub-libraries, each containing 10 gRNAs per gene.

Optimized for cleavage activity in order to maximize the likelihood of gene knockout. Split into two sub-libraries, each containing 10 gRNAs per gene.

Focused gRNA library that targets synthetic lethal candidate and control genes with oncogenic Ras.

Contains 6 distinct subpools targeting:

• kinase
• cell cycle
• nuclear
• ribosomal
• unknown
• controls

CRISPR knockout library targeting human SLC proteins.

CRISPR knockout library targeting mouse SLC proteins.

CRISPR knockout library targeting human transmembrane transporters.

Use the genome wide CRISPR knockout libraries to target all the protein coding genes in the cattle genome.

Inducible genome-wide yeast CRISPRi library.

This retroviral CRISPR library contains gRNAs from the Yusa lab Mouse improved genome-wide library v2.

This lentiviral pooled library is a mouse genome-wide CRISPR knockout library. It is identical to the Brie library #73633, replacing Puromycin resistance with mCherry.

Lentiviral CRISPR knockout library targets human mRNA binding proteins with 10 sgRNAs/gene.

Retroviral CRISPR knockout library targets mouse mRNA binding proteins with 10 sgRNAs/gene.

Designed for genomic deletion screening of protein kinases by targeting the kinase domains.

Designed for genomic deletion screening of transcription factors by targeting the DNA binding domains.

Concise CRISPR pooled library targets the whole genome of S. pneumoniae D39V with one sgRNA per operon or gene.

Designed for genomic deletion screening of functional lncRNAs (long non-coding RNAs) using lentiviral pgRNAs (paired-guide RNAs).

Designed for genomic deletion screening of functional lncRNAs by targeting their splice sites.

Enables genome-wide CRISPR screening at high MOI through use of barcoded gRNA plasmids.

Uses CRISPR cytosine base editors for genome-scale knockout screens in human cells by perturbing gene start codons, splice sites, or by introducing premature termination codons.

Used for genome scale lentiviral CRISPR screening. Target sites were chosen in a region close to the translation initiation site.

This library is intended to be used as a technical tool for removal of mitochondrial DNA from mouse genomic DNA preparations.

This pooled CRISPR knockout library targets RNA-binding proteins with 10 guide RNAs per target.

Improved version using new design pipeline and improved gRNA scaffold.

Improved version using new design pipeline and improved gRNA scaffold.

A library of protospacer flanking sites (PFS) inserted at the 5' end of the β-lactamase gene for screening PFS preference for Cas13a/C2c2.

Delivered as two half-libraries (A and B) allowing researchers to screen with 3 or 6 gRNAs/gene, respectively.

Delivered as two half-libraries (A and B) allowing researchers to screen with 3 or 6 gRNAs/gene, respectively.

Lentiviral CRISPRa library targeting 2,989 human metabolic genes for activation of gene expression.

Lentiviral CRISPRa library targeting 2,989 mouse metabolic genes for activation of gene expression.

Lentiviral CRISPRa libraries targeting cell surface proteins and soluble factors for activation of gene expression.

CRISPR activation pooled library using p65-HSF

CRISPR activation pooled library using p65-HSF

Increases transcriptional activation of genes encoding transcription factors in combination with dCas9 activation systems, such as the SunTag and the dCas9-VP64 systems.

CRISPR activation library targeting human SLC proteins.

Increases transcriptional activation through the dCas9-SunTag-VP64 system, which enables specific recruitment of multiple copies of VP64 to the sgRNA-targeted gene.

SunTag-VP64 constructs can be used for activation and must be supplied separately.

Subpools include kinases, apoptosis, stress, trafficking, gene expression, membrane proteins, and unknown function.

Increases transcriptional activation through the dCas9-SunTag-VP64 system, which enables specific recruitment of multiple copies of VP64 to the sgRNA-targeted gene.

SunTag-VP64 constructs can be used for activation and must be supplied separately.

Subpools include kinases, apoptosis, stress, trafficking, gene expression, membrane proteins, and unknown function.

Ultra-compact, human genome-wide CRISPRa library targeting each gene with a single element encoding a dual sgRNA cassette.

The Synergistic Activation Mediator (SAM) is an engineered transcriptional activation complex for the transcriptional activation of genes encoding human membrane proteins. Consists of:

• A nucleolytically inactive Cas9-VP64 fusion
• sgRNA incorporating two MS2 RNA aptamers at the tetraloop and stem-loop 2
• MS2-P65-HSF1 plasmid which expresses the activation helper protein

The Synergistic Activation Mediator (SAM) is an engineered transcriptional activation complex for the transcriptional activation of endogenous genes. Consists of:

• A nucleolytically inactive Cas9-VP64 fusion plus sgRNA incorporating two MS2 RNA aptamers at the tetraloop and stem-loop 2
• MS2-P65-HSF1 plasmid which expresses the activation helper protein

The Synergistic Activation Mediator (SAM) is an engineered transcriptional activation complex for the transcriptional activation of endogenous genes. Consists of:

• A nucleolytically inactive Cas9-VP64 fusion
• sgRNA incorporating two MS2 RNA aptamers at the tetraloop and stem-loop 2
• MS2-P65-HSF1 plasmid which expresses the activation helper protein

The Synergistic Activation Mediator (SAM) is an engineered transcriptional activation complex for the transcriptional activation of lncRNAs. Consists of:

• A nucleolytically inactive Cas9-VP64 fusion
• sgRNA incorporating two MS2 RNA aptamers at the tetraloop and stem-loop 2
• MS2-P65-HSF1 plasmid which expresses the activation helper protein

The Synergistic Activation Mediator (SAM) is an engineered transcriptional activation complex for the transcriptional activation of endogenous genes. Consists of:

• A nucleolytically inactive Cas9-VP64 fusion
• sgRNA incorporating two MS2 RNA aptamers at the tetraloop and stem-loop 2
• MS2-P65-HSF1 plasmid which expresses the activation helper protein

These libraries contain guide RNAs to inhibit human genes involved in DNA repair and an internal edit site on the backbone. They can be used for Repair-seq experiments to map DNA repair processes and identify improved genome editing strategies.

This library contains ~93,000 unique guide RNAs targeting random positions along the genome of E. coli MG1655 with a NGG PAM motif. The library contains an average of 19 targets per gene and can be used with SpdCas9 to perform pooled CRISPRi screens.

This bacterial CRISPR inhibition library targets every small RNA and ORF of the E. coli MG1655 genome. This library has higher inhibition efficiency than the psgRNA library.

The fusion protein dCas9-KRAB can be used as the transcriptional repressor and must be supplied separately.

The fusion protein dCas9-KRAB can be used as the transcriptional repressor and must be supplied separately.

Tet-inducible yeast CRISPR inhibition library targets the genome with barcode linked guide RNAs.

Inducible CRISPR repression library targets open reading frames and non-coding RNAs in M. tuberculosis.

Inducible CRISPR repression library targets open reading frames and non-coding RNAs in M. smegmatis.

Dual sgRNA libraries containing a fixed element (non-targeting guide or EMC2 guide) and the well-established Human Genome-wide CRISPRi-v2 library in tandem.

Targets long non-coding RNA (lncRNA)-expressing genes; The fusion protein dCas9-KRAB can be used as the transcriptional repressor and must be supplied separately.

The fusion protein dCas9-KRAB can be used as the transcriptional repressor and must be supplied separately.

The fusion protein dCas9-KRAB can be used as the transcriptional repressor and must be supplied separately.

Subpools include kinases, apoptosis, stress, trafficking, gene expression, membrane proteins, and unknown function.

The fusion protein dCas9-KRAB can be used as the transcriptional repressor and must be supplied separately.

The fusion protein dCas9-KRAB can be used as the transcriptional repressor and must be supplied separately.

The fusion protein dCas9-KRAB can be used as the transcriptional repressor and must be supplied separately.

Subpools include kinases, apoptosis, stress, trafficking, gene expression, membrane proteins, and unknown function.

Ultra-compact, human genome-wide CRISPRi library targeting each gene with a single element encoding a dual sgRNA cassette.

This library targets ~4,000 E. coli genes with ~15 gRNAs per gene and includes ~10,000 gRNAs targeting intergenic regions. Additionally, 2,400 gRNAs without any significant hit across the E. coli genome are provided as a negative control. The library is divided into 5 sublibraries defined by biological categories, a negative control (NC) control library, and a custom test library targeting 86 genes (50 gRNAs per gene).