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Link to original content: http://pubmed.ncbi.nlm.nih.gov/39498999/
Molecular Identification, Histopathology and Antibiotic Susceptibility Profiling of Aeromonas veronii Isolated from Oreochromis niloticus in Bangladesh - PubMed Skip to main page content
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. 2024 Nov;10(6):e70103.
doi: 10.1002/vms3.70103.

Molecular Identification, Histopathology and Antibiotic Susceptibility Profiling of Aeromonas veronii Isolated from Oreochromis niloticus in Bangladesh

Sayed Mashequl Bari  1 , Mohammad Muttakinul Islam  2 , Aktia Amina  2 , Marufa Khatun  1 , A M Shahabuddin  3
Affiliations

Molecular Identification, Histopathology and Antibiotic Susceptibility Profiling of Aeromonas veronii Isolated from Oreochromis niloticus in Bangladesh

Sayed Mashequl Bari et al. Vet Med Sci. 2024 Nov.

Abstract

Background: Tilapia (Oreochromis niloticus) is the most widely cultured freshwater fish species in Bangladesh and worldwide. However, commercial tilapia culture systems face increasing challenges from bacterial infections.

Objectives: The objective of this study was to identify the bacterial isolates from infected tilapia in an intensive cage culture farm located along the Shitalakshya River in Bangladesh.

Methods: Infected fish samples were collected and underwent comprehensive clinical and post-mortem investigations, followed by phenotypic, biochemical and molecular identification of the bacterial isolates, as well as histopathological and antibiotic susceptibility examinations.

Results: Phenotypic and biochemical characterization showed similarities of the -collected isolates with Aeromonas veronii. Moreover, molecular analysis of the bacterial conserved region 16S rRNA also confirmed these isolates as A. veronii. The analysed 16S rRNA sequence (GenBank accession no. PP832815) showed a close relationship (100% identity) with A. veronii from China (GenBank accession no. MT071624) in the NCBI BLAST search, and in the phylogenetic tree, they grouped in a single clade. This close genetic relationship is also supported by the low genetic distance between the isolates. Histopathological analysis revealed gross pathological changes like necrosis, hypertrophy and inflammation in muscle tissues. The isolates were found to be sensitive to multiple antibiotics but resistant to trimethoprim and sulphamethoxazole.

Conclusion: This study investigated the presence of A. veronii infection in tilapia (O. niloticus) in an intensive cage culture farm in Bangladesh.

Keywords: Aeromonas veronii; Tilapia; antibiotic susceptibility test; histopathology; molecular identification.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

FIGURE 1
FIGURE 1
Geographical map of the sampling area (software: QGIS 3.38). Black points on the map represent the sampling points on the country, division and district maps.
FIGURE 2
FIGURE 2
Clinical observation of an infected Tilapia sample. The white arrow indicates (A) exophthalmia in the eye, (B) abdominal distension and black spot on body surface, (C) necrotic spleen, liver and intestine.
FIGURE 3
FIGURE 3
Colony morphology of Aeromonas veronii. (A) A. veronii produces white colonies on MacConkey agar. (B) Pale smooth, convex and rounded colonies on blood agar.
FIGURE 4
FIGURE 4
Phylogenetic tree derived from 16S rRNA gene sequences showing relationships of strain PP831815 to all other described closely related species of the same genus Aeromonas and out‐group Bacillus. The tree was reconstructed using the neighbour‐joining method. Percentage bootstrap values (1000 replicates) are shown at each branch point. The scale bar 0.002 represents substitutions per nucleotide position. The black squared dot represents the studied isolate.
FIGURE 5
FIGURE 5
Histopathological alterations (muscle) in diseased Tilapia (Oreochromis niloticus). (A) Cellular degeneration and atrophy of muscle fibres (H&E, ×10). (B) Loss of cellularity, significant size variation (H&E, ×10). (C) Lymphocytic infiltration the arrow showing monocyte, intermuscular oedema (H&E, ×4). (D) Mononuclear inflammatory cells in muscle (black head arrow) (H&E, ×4).
See this image and copyright information in PMC

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