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Antibiotic Resistance Profile and Bio-Control of Multidrug-Resistant Escherichia coli Isolated from Raw Milk in Vietnam Using Bacteriophages - PubMed Skip to main page content
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. 2024 Jun 9;13(6):494.
doi: 10.3390/pathogens13060494.

Antibiotic Resistance Profile and Bio-Control of Multidrug-Resistant Escherichia coli Isolated from Raw Milk in Vietnam Using Bacteriophages

Hoang Minh Duc  1 , Tran Thi Khanh Hoa  1 , Cam Thi Thu Ha  1 , Le Van Hung  2 , Nguyen Van Thang  2 , Hoang Minh Son  3 , Gary A Flory  4
Affiliations

Antibiotic Resistance Profile and Bio-Control of Multidrug-Resistant Escherichia coli Isolated from Raw Milk in Vietnam Using Bacteriophages

Hoang Minh Duc et al. Pathogens. .

Abstract

E. coli is an important zoonotic pathogen capable of causing foodborne illness and bovine mastitis. Bacteriophages have been increasingly considered a promising tool to control unwanted bacteria. The aim of this study is to determine the antibiotic resistance profile of E. coli isolated from raw milk and the efficacy of phage in controlling multidrug-resistant E. coli in raw milk. Antibiotic susceptibility testing showed the highest resistance rates of E. coli isolates to co-trime (27.34%) and ampicillin (27.34%), followed by streptomycin (25.18%), tetracycline (23.02%), and the lowest resistance rates to ciprofloxacin, gentamycin, and ceftazidime, all at a rate of 2.16%. All isolates were susceptible to meropenem. Of the 139 E. coli isolates, 57 (41.01%) were resistant to at least one antibiotic, and 35 (25.18%) were classified as MDR strains. Molecular characterization indicated that 5 (3.6%) out of the 139 isolates were STEC strains carrying stx1 gene. Seven (5.04%) isolates were phenotypically identified as ESBLEC, and four isolates (2.88%) were resistant to colistin. The results of the genotypic test revealed that four out of seven ESBLEC strains carried both blaTEM and blaCTX-M-1, two harbored blaTEM, and one possessed blaCTX-M-1, while mcr-1 was detected in all four colistin-resistant E. coli isolates. In particular, one isolated E. coli strain (EM148) was determined to be a multidrug-resistant strain simultaneously carrying blaTEM, blaCTX-M-1, and mcr-1. A total of eight phages were successfully recovered from raw milk. The application of phage PEM3 significantly reduced viable counts of multidrug-resistant host EM148 in raw milk by at least 2.31 log CFU/mL at both 24 °C and 4 °C.

Keywords: E. coli; antibiotic resistance; bacteriophages; multidrug resistance.

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Conflict of interest statement

Author Gary A. Flory was employed by the company G.A. Flory Consulting. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
The clear plaques of phage PEM3.
Figure 2
Figure 2
One-step growth curves of phages PEM3 in E. coli EM148. L, latent period; B, burst. Error bars show standard deviations.
Figure 3
Figure 3
Effects of temperature (a), pH (b), and NaCl (c) on the stability of phage PEM3. Error bars show standard deviations.
Figure 4
Figure 4
Effect of phage PEM3 on the viability of E. coli EM148 in LB broth stored at 24 °C (a) and 4 °C (b). E. coli EM148 was inoculated in 5 mL of LB broth at a final concentration of 105 CFU/mL without (dashed line) and with phage PEM3 at 108 PFU/mL (solid line). Error bars show standard deviations.
Figure 5
Figure 5
Effect of phage PEM3 on the viability of E. coli EM148 in raw milk stored at 24 °C (a) and 4 °C (b). E. coli EM148 was inoculated in 5 mL of raw milk at a final concentration of 105 CFU/mL without (dashed line) and with phage PEM3 at 108 PFU/mL (solid line). Error bars show standard deviations.
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