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Chromosomal mapping of repetitive DNA and retroelement sequences and its implications for the chromosomal evolution process in Ctenoluciidae (Characiformes) - PubMed Skip to main page content
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. 2024 May 30;24(1):72.
doi: 10.1186/s12862-024-02262-x.

Chromosomal mapping of repetitive DNA and retroelement sequences and its implications for the chromosomal evolution process in Ctenoluciidae (Characiformes)

José Francisco de Sousa E Souza  1 , Erika Milena Corrêa Guimarães  2 , Vanessa Susan Pinheiro Figliuolo  2 , Simone Cardoso Soares  2 , Marcelo de Bello Cioffi  3 , Francisco de Menezes Cavalcante Sassi  3 , Eliana Feldberg  2
Affiliations

Chromosomal mapping of repetitive DNA and retroelement sequences and its implications for the chromosomal evolution process in Ctenoluciidae (Characiformes)

José Francisco de Sousa E Souza et al. BMC Ecol Evol. .

Abstract

Ctenoluciidae is a Neotropical freshwater fish family composed of two genera, Ctenolucius (C. beani and C. hujeta) and Boulengerella (B. cuvieri, B. lateristriga, B. lucius, B. maculata, and B. xyrekes), which present diploid number conservation of 36 chromosomes and a strong association of telomeric sequences with ribosomal DNAs. In the present study, we performed chromosomal mapping of microsatellites and transposable elements (TEs) in Boulengerella species and Ctenolucius hujeta. We aim to understand how those sequences are distributed in these organisms' genomes and their influence on the chromosomal evolution of the group. Our results indicate that repetitive sequences may had an active role in the karyotypic diversification of this family, especially in the formation of chromosomal hotspots that are traceable in the diversification processes of Ctenoluciidae karyotypes. We demonstrate that (GATA)n sequences also accumulate in the secondary constriction formed by the 18 S rDNA site, which shows consistent size heteromorphism between males and females in all Boulengerella species, suggesting an initial process of sex chromosome differentiation.

Keywords: Chromosomal hotspots; Neotropical fish; Pike characins; Sex chromosomes.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
Chromosomal mapping of the TE Rex1 in Ctenoluciidae: a) Boulengerella cuvieri, b) B. lucius, c) B. maculata, d) Ctenolucius hujeta
Fig. 2
Fig. 2
Chromosomal mapping of the TE Rex3 in Ctenoluciidae: a) Boulengerella cuvieri, b) B. lateristriga, c) B. lucius, d) B. maculata, e) Ctenolucius hujeta
Fig. 3
Fig. 3
Chromosomal mapping of TE Rex6 in Ctenoluciidae: a) Boulengerella cuvieri, b) B. lateristriga, c) B. maculate
Fig. 4
Fig. 4
Karyotypes of males and females of Boulengerella lateristriga, B. maculata, B. lucius, and B. cuvieri, submitted to FISH with the probe (GATA)7. The (GATA)7 signals are shown in rhodamine (red) and chromosomes are counterstained with DAPI (blue)
Fig. 5
Fig. 5
Chromosomal mapping of distinct microsatellite sequences in Boulengerella cuvieri, B. lateristriga, B. lucius and B. maculata. Metaphasic plates hybridized with the microsatellite probes d(GAG)10; d(CAA)10; d(CAC)10 and d(CAT)10 (red), revealing the distribution pattern on columns 1–4, respectively. Arrows indicate the position of chromosome pair 18
Fig. 6
Fig. 6
Chromosomal mapping of distinct microsatellite sequences in Ctenolucius hujeta. Karyotype of males and females hybridized with microsatellites d(GATA)7 (upper section). Metaphasic plates hybridized with microsatellite probes d(GAG)10; d(CAA)10; d(CAC)10; d(CAT)10, respectively, showing the patter of those SSR at chromosomes (lower section)
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