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. 2024 May;291(9):1889-1891.
doi: 10.1111/febs.17129. Epub 2024 Apr 5.

Primase and polymerase α tango to make an RNA-DNA hybrid primer

Zuanning Yuan  1 , Huilin Li  1
Affiliations

Primase and polymerase α tango to make an RNA-DNA hybrid primer

Zuanning Yuan et al. FEBS J. 2024 May.

Abstract

Several recent cryo-electron microscopy (cryo-EM) studies about the eukaryotic primosome, including the human primosome described by Yin et al. in this issue, have uncovered the structural intricacies between the RNA primase and the DNA polymerase. These studies show that these two partners tango on DNA to synthesize a hybrid primer composed of ~ 10 nucleotide (nt) RNA and ~ 10-nt DNA. They reveal key intermediate steps involved in this process; from the self-inhibited apo state to the initiation of RNA primer synthesis, RNA primer handover to the polymerase, primer elongation by polymerase, and finally, primer termination and release. Remarkably, the polymerase domain orchestrates all major steps during primer synthesis.

Keywords: DNA polymerase; RNA; cryo‐EM; primase; primer initiation.

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Conflict of interest statement

Conflicts of interest: The authors declare no conflict of interest.

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