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Link to original content: http://pubmed.ncbi.nlm.nih.gov/37239430/
Stacking Multiple Genes Improves Resistance to Chilo suppressalis, Magnaporthe oryzae, and Nilaparvata lugens in Transgenic Rice - PubMed Skip to main page content
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. 2023 May 12;14(5):1070.
doi: 10.3390/genes14051070.

Stacking Multiple Genes Improves Resistance to Chilo suppressalis, Magnaporthe oryzae, and Nilaparvata lugens in Transgenic Rice

Bai Li  1 , Zhongkai Chen  1 , Huizhen Chen  2 , Chunlei Wang  1 , Liyan Song  1 , Yue Sun  1 , Yicong Cai  1 , Dahu Zhou  1 , Linjuan Ouyang  1 , Changlan Zhu  1 , Haohua He  1 , Xiaosong Peng  1
Affiliations

Stacking Multiple Genes Improves Resistance to Chilo suppressalis, Magnaporthe oryzae, and Nilaparvata lugens in Transgenic Rice

Bai Li et al. Genes (Basel). .

Abstract

The ability of various pests and diseases to adapt to a single plant resistance gene over time leads to loss of resistance in transgenic rice. Therefore, introduction of different pest and disease resistance genes is critical for successful cultivation of transgenic rice strains with broad-spectrum resistance to multiple pathogens. Here, we produced resistance rice lines with multiple, stacked resistance genes by stacking breeding and comprehensively evaluated their resistance to Chilo suppressalis (striped rice stemborer), Magnaporthe oryzae (rice blast), and Nilaparvata lugens (brown planthopper) in a pesticide-free environment. CRY1C and CRY2A are exogenous genes from Bacillus thuringiensis. Pib, Pikm, and Bph29 are natural genes in rice. CH121TJH was introduced into CRY 1C, Pib, Pikm, and Bph29. CH891TJH and R205XTJH were introduced into CRY 2A, Pib, Pikm, and Bph29. Compared with those observed in their recurrent parents, CH121TJH significantly increased the mortality of borers. The other two lines CH891TJH and R205XTJH are the same result. Three lines introduction of Pib and Pikm significantly reduced the area of rice blast lesions, and introduction of Bph29 significantly reduced seedling mortality from N. lugens. Introduction of the exogenous genes had relatively few effects on agronomic and yield traits of the original parents. These findings suggest that stacking of rice resistance genes through molecular marker-assisted backcross breeding can confer broad spectrum and multiple resistance in differently genetic backgrounds.

Keywords: Chilo suppressalis; Magnaporthe oryzae; Nilaparvata lugens; rice blast; transgenic rice.

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Conflict of interest statement

All authors declare that they have no conflict of interest.

Figures

Figure 1
Figure 1
PCR amplification products from donor parents, recurrent parents, and transgenic rice with polymerized resistance genes. The names of the plants in each tunnel are marked on the diagram. M is DL2000 Maker. (A). Cry1C and Cry2A. (B). Pib and Lys145. (C). Bph29. (D). Pikm1 and Pikm2. The primer of A1–A3 is CRY 1C. The primer of A4–A8 is CRY 2A. The primer of B1, B3, B5, B7, B9, B11, and B13 is Pib. The primer of B2, B4, B6, B8, B10, B12, and B14 is Lys145. The primer of C1–C7 is Bph29. The primer of D1, D3, D5, D7, D9, D11, and D13 is Dkm1. The primer of D2, D4, D6, D8, D10, D12, and D14 is Dkm2.
Figure 2
Figure 2
Flow chart depicting the breeding process of polymeric resistance gene lines. CH121TJH was produced by backcrossing MH63 (Cry1C) as a donor parent with CH121 as a recurrent parent. CH891TJH was produced by backcrossing MH63 (Cry2A) with CH891, and R205XTJH was produced by backcrossing MH63 (Cry2A) with R205X. ‘×’ means crossbreeding, ‘⊗’ means self-cross breeding.
Figure 3
Figure 3
The chromosomal distributions of the 560 microsatellite markers used for genotyping CH121TJH, CH891TJH, R205XTJH, and their recurrent parent. Blue, green, and red bars represent the homozygous markers for the PSL genome in the three restorer lines CH121TJH, CH891TJH and R205XTJH, respectively. The remaining markers were homozygous for the genome of the recurrent parent. The scale on the left indicates the physical position (Mb) of each marker, and the names of the microsatellite markers are on the right.
Figure 4
Figure 4
(A) Laboratory insect feeding tests using stems of CH121, CH891, R205X, CH121TJH, CH89TJH, and R205XTJH collected at the heading stage. (B) Larval mortality of Chilo suppressalis in laboratory bioassays. All tests were performed with ten replicates, and one replicate comprised 20 s instar larvae. Values are mean ± standard error.
Figure 5
Figure 5
(A) Proportion of uninfected leaf area in multiple rice lines after spraying with an M. oryzae spore suspension (** p < 0.01, LSD test). (B) Leaf phenotypes after Magnaporthe oryzae inoculation.
Figure 6
Figure 6
Nilaparvata lugens resistance in various parental and transgenic rice lines at the seedling stage. (A) CH121TJH. (B) CH891TJH. (C) R205X. (D) Seedling mortality. Medians are indicated by solid bold lines. * 0.01 < p < 0.05 (LSD), ** p < 0.01 (LSD). (E) Pie chart showing the resistance levels of various lines to N. lugens.
Figure 7
Figure 7
Comparison of eight agronomic traits in CH121TJH, CH891TJH, and R205XTJH with those of their original parents. (A) Plant height (cm). (B) Total grain number. (C) Effective panicle number. (D) Panicle length (cm). (E) Seed setting rate. (F) Yield per plant (g). (G) Number of solid grains. (H) 1000-grain weight (g). ** p < 0.01 (LSD),* 0.01 < p < 0.05 (LSD), ns means none otherness.
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References

    1. Caron P., Ferrero de Loma-Osorio G., Ferroni M., Lehmann B., Mettenleiter T.C., Sokona Y. Global Food Security: Pool Collective Intelligence. Nature. 2022;612:631. doi: 10.1038/d41586-022-04471-0. - DOI - PubMed
    1. Litsinger J.A., Canapi B.L., Bandong J.P., Cruz C.D., Apostol R.F., Pantua P.C., Lumaban M.D., Alviola A.L., Raymundo F., Libetario E.M., et al. Rice Crop Loss from Insect Pests in Wetland and Dryland Environments of Asia with Emphasis on the Philippines. Int. J. Trop. Insect Sci. 2011;8:677–692. doi: 10.1017/S1742758400022785. - DOI
    1. Jiang Y., Pan S., Cai M., Li C., Zhan M., Wang J., Mohamed I., Cao C. Assessment of yield advantages of Bt-MH63 with cry1C* or cry2A* genes over MH63 (Oryza sativa L.) under different pest control modes. Field Crops Res. 2014;155:153–158. doi: 10.1016/j.fcr.2013.09.011. - DOI
    1. Khush G.S., Jena K.K. Advances in Genetics, Genomics and Control of Rice Blast Disease. Conf. Proc. 2009;1:1–10. doi: 10.1007/978-1-4020-9500-9_1. - DOI
    1. Pham V.D., Cabunagan R.C., Cabauatan P.Q., Choi H.S., Choi I.R., Ho V.C., Nguyen H.H. Yellowing syndrome of rice: Etiology, current status and future challenges. Omonrice. 2007;15:94–101.

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This research was supported by the Major Project supported by the Major Project of Jiangxi Provincial Department of Science and Technology (S2016NYZPF0256), the Major Project supported by Breeding New Varieties of the National Transgene of China (2016ZX08001-001-005), Jiangxi Provincial Natural Science Foundation of China (20202BAB215001). District Science Foundation Project of National Natural Science Foundation (32060477). Jiangxi Natural Science Foundation (20202BAB205007). The Major Project of Jiangxi Provincial Department of Science and Technology (2016NYZPF0256).