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Anti-diabetic activities of phenolic compounds of Alternaria sp., an endophyte isolated from the leaves of desert plants growing in Egypt - PubMed Skip to main page content
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. 2022 Sep 1;12(38):24935-24945.
doi: 10.1039/d2ra02532a. eCollection 2022 Aug 30.

Anti-diabetic activities of phenolic compounds of Alternaria sp., an endophyte isolated from the leaves of desert plants growing in Egypt

Ahmed Elbermawi  1 , Ahmed R Ali  2 , Yhiya Amen  1 , Ahmed Ashour  1 , Kadria F Ahmad  1 , El-Sayed S Mansour  1 , Ahmed F Halim  1
Affiliations

Anti-diabetic activities of phenolic compounds of Alternaria sp., an endophyte isolated from the leaves of desert plants growing in Egypt

Ahmed Elbermawi et al. RSC Adv. .

Abstract

Six phenolic compounds (talaroflavone (1), alternarienoic acid (2), altenuene (3), altenusin (4), alternariol (5), and alternariol-5-O-methyl ether (6)) were isolated from the solid rice culture media of Alternaria sp., an endophyte isolated from the fresh leaves of three desert plants, Lycium schweinfurthii Dammer (Solanaceae), Pancratium maritimum L. (Amaryllidaceae) and Cynanchum acutum L. (Apocynaceae). Compounds 2, 3, and 4 exhibited potent α-glucosidase and lipase inhibitory activities suggesting that they might act as naturally occurring anti-diabetic candidates. The same compounds showed potent binding in the active site for both enzymes with desirable pharmacokinetic properties. The isolated bioactive compounds were not exclusive to a certain host plant which reveals the dominant ecological standpoints for consequent optimization. This could lead to a cost-effective and reproducible yield applicable to commercial scale-up.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Fig. 1
Fig. 1. Structures of the isolated compounds from Alternaria sp.
Fig. 2
Fig. 2. Tracking the isolated compounds in different Alternaria sp. culture media extracts, HPLC chromatograph of mix of compounds (blue), the EtOAc extract of Alternaria sp. from L. schweinfurthii (pink), C. acutum (brown) and P. maritimum (green).
Fig. 3
Fig. 3. 2D Interaction diagram for compounds 1–6 and acarbose with alpha-glucosidase enzyme (PDB id: 5ZCC).
Fig. 4
Fig. 4. 2D Interaction diagram for compounds 1–6 and orlistat with pancreatic lipase enzyme (PDB id: 1LPB).
Fig. 5
Fig. 5. PLIF analysis for compounds 1–6 with: (a) acarbose and alpha-glucosidase enzyme, (b) orlistat with pancreatic lipase enzyme.
Fig. 6
Fig. 6. Interaction surface based on van der Waals radius with isolated compounds (green-colored regions are located deep within the active site and red-colored areas are solvent exposed).
Fig. 7
Fig. 7. Predicting electrostatically favorable regions for isolated compounds aligned over control compounds via interaction potential surface (lipophilic regions are colored green, positively charged regions (donor areas) are blue in color, and negative regions (acceptor) are colored in red).
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