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Link to original content: http://pubmed.ncbi.nlm.nih.gov/30283073/
A Comparative Study of Hummingbirds and Chickens Provides Mechanistic Insight on the Histidine Containing Dipeptide Role in Skeletal Muscle Metabolism - PubMed Skip to main page content
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. 2018 Oct 3;8(1):14788.
doi: 10.1038/s41598-018-32636-3.

A Comparative Study of Hummingbirds and Chickens Provides Mechanistic Insight on the Histidine Containing Dipeptide Role in Skeletal Muscle Metabolism

E Dolan  1 , B Saunders  1 , W S Dantas  1 , I H Murai  1 , H Roschel  1 , G G Artioli  1 , R Harris  2 , J E P W Bicudo  3 , C Sale  4 , B Gualano  5
Affiliations

A Comparative Study of Hummingbirds and Chickens Provides Mechanistic Insight on the Histidine Containing Dipeptide Role in Skeletal Muscle Metabolism

E Dolan et al. Sci Rep. .

Abstract

Histidine containing dipeptides (HCDs) have numerous ergogenic and therapeutic properties, but their primary role in skeletal muscle remains unclear. Potential functions include pH regulation, protection against reactive oxygen/nitrogen species, or Ca2+ regulation. In recognition of the challenge of isolating physiological processes in-vivo, we employed a comparative physiology approach to investigate the primary mechanism of HCD action in skeletal muscle. We selected two avian species (i.e., hummingbirds and chickens), who represented the extremes of the physiological processes in which HCDs are likely to function. Our findings indicate that HCDs are non-essential to the development of highly oxidative and contractile muscle, given their very low content in hummingbird skeletal tissue. In contrast, their abundance in the glycolytic chicken muscle, indicate that they are important in anaerobic bioenergetics as pH regulators. This evidence provides new insights on the HCD role in skeletal muscle, which could inform widespread interventions, from health to elite performance.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Figure 1
Figure 1
Characteristics of the Hummingbird & Chicken M. Pectoralis,,,,,,,,–.
Figure 2
Figure 2
Histidine Containing Dipeptide (HCD) Content of Hummingbird and Chicken. **p < 0.01 from hummingbird m. pectoralis; ƴƴ p < 0.01 from chicken m. vastus lateralis. Hummingbird m. pectoralis n = 5; chicken m. vastus lateralis n = 6; chicken m. pectoralis n = 6.
Figure 3
Figure 3
Characteristics of Hummingbird M. Pectoralis (HP), Chicken M. Vastus Lateralis (CV) and Chicken M. Pectoralis (CP). Panel (A) COX IV content; Panel (B) LDH content; Panel (C) Superoxide Dismutase (SOD) Activity; Panel (D) Muscle buffering capacity (βm) represented with and without HCD contribution. **p < 0.01 from hummingbird m. pectoralis; ƴƴ p < 0.01 from chicken m. vastus lateralis; Hummingbird m. pectoralis n = 5; chicken m. vastus lateralis n = 6; chicken m. pectoralis n = 6. The bands shown in panels A and B were loaded in the order described and were cropped from the same location and gel. Full-length blots are presented in Supplementary File 1.
Figure 4
Figure 4
Coefficient of Determination Between Total HCD content and Superoxide Dismutase (SOD) Activity Panel (A), and Total HCD Content and βm Panel (B). Analyses are based on 17 samples, i.e., Hummingbird m. pectoralis n = 5; chicken m. vastus lateralis n = 6; chicken m. pectoralis n = 6.
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