iBet uBet web content aggregator. Adding the entire web to your favor.
iBet uBet web content aggregator. Adding the entire web to your favor.



Link to original content: http://pubmed.ncbi.nlm.nih.gov/24468282/
Expression of proteins associated with adipocyte lipolysis was significantly changed in the adipose tissues of the obese spontaneously hypertensive/NDmcr-cp rat - PubMed Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2014 Jan 27;6(1):8.
doi: 10.1186/1758-5996-6-8.

Expression of proteins associated with adipocyte lipolysis was significantly changed in the adipose tissues of the obese spontaneously hypertensive/NDmcr-cp rat

Jie Chang, Shinji Oikawa, Hitoshi Iwahashi, Emiko Kitagawa, Ichiro Takeuchi, Masao Yuda, Chieko Aoki, Yoshiji Yamada, Gaku Ichihara, Masashi Kato, Sahoko Ichihara  1
Affiliations

Expression of proteins associated with adipocyte lipolysis was significantly changed in the adipose tissues of the obese spontaneously hypertensive/NDmcr-cp rat

Jie Chang et al. Diabetol Metab Syndr. .

Abstract

Background: The etiology of the metabolic syndrome is complex, and is determined by the interplay of both genetic and environmental factors. The present study was designed to identify genes and proteins in the adipose tissues with altered expression in the spontaneously hypertensive/NIH -corpulent rat, SHR/NDmcr-cp (CP) and to find possible molecular targets associated with the pathogenesis or progression of obesity related to the metabolic syndrome.

Methods: We extracted RNAs and proteins from the epididymal adipose tissues in CP, SHR/Lean (Lean), and Wistar Kyoto (WKY) rats and performed microarray analysis and two-dimensional difference in gel electrophoresis (2D-DIGE) linked to a matrix-assisted laser desorption ionization time-of-flight tandem mass spectrometry (MALDI-TOF/TOF MS).

Results: The results showed different mRNA and protein expression levels in the adipose tissue: oligo DNA microarray identified 33 genes that were significantly (P < 0.01) up-regulated and 17 genes significantly down-regulated in CP compared with WKY and Lean rats at both 6 and 25 weeks of age. The affected genes-proteins were associated with lipolytic enzymes stimulated by peroxisome proliferator-activated receptor (PPAR) signaling. Further analysis using the 2D-DIGE connected with MALDI-TOF/TOF analysis, the expression of monoglyceride lipase (MGLL) was significantly up-regulated and that of carboxylesterase 3 (CES3) was significantly down-regulated in 6- and 25-week-old CP compared with age-matched control (WKY and Lean rats).

Conclusions: Our results suggest the possible involvement of proteins associated with adipocyte lipolysis in obesity related to the metabolic syndrome.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Body weight and plasma biochemical data. (A) Body weight, (B) epididymal adipose tissue weight, and plasma levels of (C) triglyceride and (D) non-fasting glucose in WKY, Lean, and CP rats at 6 and 25 weeks of age. Data are mean ± SEM of six animals per group. *P < 0.05, versus the corresponding value for WKY. WKY; Wistar Kyoto rats, Lean; spontaneously hypertensive rats (SHR/lean), CP; SHR/NDmcr-cp (cp/cp) rats.
Figure 2
Figure 2
Representative 2D-DIGE image of adipose lysates from 25-week-old Lean and CP rats. (A) The proteins (40 μg each) were labeled with Cy3 and Cy5 dyes, mixed and subjected to 2D-DIGE analysis. We selected significantly different spots of 6- and 25-week-old CP compared with age-matched control (WKY and Lean) rats. The expression level was quantified in each strain at 6 (B) and 25 weeks of age (C) and expressed as mean ± SEM of four rats per group. *P < 0.05, versus the corresponding value for WKY or Lean rats. WKY; Wistar Kyoto rats, Lean; spontaneously hypertensive rats (SHR/lean), CP; SHR/NDmcr-cp (cp/cp) rats.
Figure 3
Figure 3
Gene expression of the identified proteins in WKY, Lean, and CP rats at 6 and 25 weeks of age. The mRNA levels of Spin2b, AHSG, CES3, MGLL, and PDHB1 in the adipose tissues were determined by quantitative RT-PCR analysis and expressed relative to that of β-actin. Quantitative data are expressed relative to the values for WKY. Data are mean ± SEM of six animals per group. *P < 0.05, versus the corresponding value for WKY. WKY; Wistar Kyoto rats, Lean; spontaneously hypertensive rats (SHR/lean), CP; SHR/NDmcr-cp (cp/cp) rats.
Figure 4
Figure 4
The amounts of AHSG, CES3, and MGLL in WKY, Lean, and CP rats at 6 and 25 weeks of age. (A) Representative immunoblots of each protein. (B) The amount of each protein quantitated relative to the amount of β-actin and expressed relative to the value of WKY. Data are mean ± SEM of six animals per group. *P < 0.05, versus the corresponding value for WKY. WKY; Wistar Kyoto rats, Lean; spontaneously hypertensive rats (SHR/lean), CP; SHR/NDmcr-cp (cp/cp) rats.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Kopelman PG. Obesity as a medical problem. Nature. 2000;404:635–643. - PubMed
    1. Wilson PWF, D’Agostino RB, Parise H. et al.Metabolic syndrome as a precursor of cardiovascular disease and type 2 diabetes mellitus. Circulation. 2005;112:3066–3072. doi: 10.1161/CIRCULATIONAHA.105.539528. - DOI - PubMed
    1. McLaughlin T, Abbasi F, Lamendola C. et al.Heterogeneity in the prevalence of risk factors for cardiovascular disease and type 2 diabetes mellitus in obese individuals. Arch Intern Med. 2007;167:642–648. doi: 10.1001/archinte.167.7.642. - DOI - PubMed
    1. Gohdes D, Amundson H, Oser CS. et al.How are we diagnosing cardiometabolic risk in primary care settings? Prim Care Diabetes. 2009;3:29–35. doi: 10.1016/j.pcd.2008.12.002. - DOI - PubMed
    1. Morricone L, Malavazos AE, Coman C. et al.Echocardiographic abnormalities in normotensive obese patients: relationship with visceral fat. Obes Res. 2002;10:489–498. doi: 10.1038/oby.2002.67. - DOI - PubMed

LinkOut - more resources