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Link to original content: http://pubmed.ncbi.nlm.nih.gov/23429197/
5-bp Classical Satellite DNA Loci from Chromosome-1 Instability in Cervical Neoplasia Detected by DNA Breakage Detection/Fluorescence in Situ Hybridization (DBD-FISH) - PubMed Skip to main page content
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. 2013 Feb 19;14(2):4135-47.
doi: 10.3390/ijms14024135.

5-bp Classical Satellite DNA Loci from Chromosome-1 Instability in Cervical Neoplasia Detected by DNA Breakage Detection/Fluorescence in Situ Hybridization (DBD-FISH)

Elva I Cortés-Gutiérrez  1 , Brenda L Ortíz-Hernández, Martha I Dávila-Rodríguez, Ricardo M Cerda-Flores, José Luis Fernández, Carmen López-Fernández, Jaime Gosálvez
Affiliations

5-bp Classical Satellite DNA Loci from Chromosome-1 Instability in Cervical Neoplasia Detected by DNA Breakage Detection/Fluorescence in Situ Hybridization (DBD-FISH)

Elva I Cortés-Gutiérrez et al. Int J Mol Sci. .

Abstract

We aimed to evaluate the association between the progressive stages of cervical neoplasia and DNA damage in 5-bp classical satellite DNA sequences from chromosome-1 in cervical epithelium and in peripheral blood lymphocytes using DNA breakage detection/fluorescence in situ hybridization (DBD-FISH). A hospital-based unmatched case-control study was conducted in 2011 with a sample of 30 women grouped according to disease stage and selected according to histological diagnosis; 10 with low-grade squamous intraepithelial lesions (LG-SIL), 10 with high-grade SIL (HG-SIL), and 10 with no cervical lesions, from the Unidad Medica de Alta Especialidad of The Mexican Social Security Institute, IMSS, Mexico. Specific chromosome damage levels in 5-bp classical satellite DNA sequences from chromosome-1 were evaluated in cervical epithelium and peripheral blood lymphocytes using the DBD-FISH technique. Whole-genome DNA hybridization was used as a reference for the level of damage. Results of Kruskal-Wallis test showed a significant increase according to neoplastic development in both tissues. The instability of 5-bp classical satellite DNA sequences from chromosome-1 was evidenced using chromosome-orientation FISH. In conclusion, we suggest that the progression to malignant transformation involves an increase in the instability of 5-bp classical satellite DNA sequences from chromosome-1.

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Figures

Figure 1
Figure 1
Representative set of cervical epithelium after DNA breakage detection/fluorescence in situ hybridization (DBD-FISH) in control women, with LG-SIL, and HG-SIL, using whole-genome probe (A to C respectively) and 5-bp classical satellite DNA sequences from chromosome-1 probes (A′ to C′ respectively) labeled with biotin (red) and counterstained with DAPI (blue).
Figure 2
Figure 2
Representative set of peripheral blood lymphocytes after DBD-FISH in control women, with LG-SIL and HG-SIL, using whole-genome (A to C respectively), and 5-bp classical satellite DNA sequences from chromosome-1 probes (A′ to C′ respectively) labeled with biotin (red) and counterstained with DAPI (blue).
Figure 3
Figure 3
Chromosome-Orientation Fluorescence in situ Hybridization (CO-FISH) using Exo III DNA-selective DNA breakage in bromodeoxyuridine-treated cells and a human 5-bp repetitive DNA probe (chromosomes 1, 9 and 16) in patients with cervical neoplasia (A). Note the sister chromatid exchange affecting the specific DNA family in selected chromosome-1sequences (B right) respect to chromosome-1 without sister chromatid exchange in this region (B left).
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