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Link to original content: http://pubmed.ncbi.nlm.nih.gov/21261862/
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. 2008 Sep;1(5):425-31.
doi: 10.1111/j.1751-7915.2008.00045.x.

Erythrophore cell response to food-associated pathogenic bacteria: implications for detection

Janine R Hutchison  1 , Stephanie R Dukovcic, Karen P Dierksen, Calvin A Carlyle, Bruce A Caldwell, Janine E Trempy
Affiliations

Erythrophore cell response to food-associated pathogenic bacteria: implications for detection

Janine R Hutchison et al. Microb Biotechnol. 2008 Sep.

Abstract

Cell-based biosensors have been proposed for use as function-based detectors of toxic agents. We report the use of Betta splendens chromatophore cells, specifically erythrophore cells, for detection of food-associated pathogenic bacteria. Evaluation of erythrophore cell response, using Bacillus spp., has revealed that this response can distinguish pathogenic Bacillus cereus from a non-pathogenic B. cereus ΔplcR deletion mutant and a non-pathogenic Bacillus subtilis. Erythrophore cells were exposed to Salmonella enteritidis, Clostridium perfringens and Clostridium botulinum. Each bacterial pathogen elicited a response from erythrophore cells that was distinguished from the corresponding bacterial growth medium, and this observed response was unique for each bacterial pathogen. These findings suggest that erythrophore cell response has potential for use as a biosensor in the detection and toxicity assessment for food-associated pathogenic bacteria.

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Figures

Figure 1
Figure 1
B. splendens erythrophore cell response to Bacillus spp. Erythrophore cells appearance at Time 0 (left) and Time 20 min (right) upon exposure to: (A) BHI medium, (B) B. subtilis 1A1, (C) B. cereus ATCC 49064. Images are at 100×, the size bar represents a length of 100 µm. (D) Graphical display of erythrophore cell pigment area change in response to Bacillus spp. A negative change in pigment area is indicative of pigment aggregation in erythrophore cells whereas a positive change in pigment area represents pigment dispersion. (▴) BHI medium; (●) B. subtilis 1A1; (◆) B. cereus ATCC 49064; (▪) B. cereus ATCC 14579; (□) B. cereus ΔplcR deletion mutant strain that is a derivative of ATCC 14579. Data represent the mean values of three trials.
Figure 2
Figure 2
B. splendens erythrophore cell response to Salmonella enteritidis and Clostridium perfringens. A negative change in pigment area is indicative of pigment aggregation whereas a positive change in pigment area represents pigment dispersion.
A. (◆) LB medium; (●) S. enteritidis ATCC 4931.
B. (▴) Duncan‐Strong sporulation medium; (▪) C. perfringens SM101.
Data represent the mean values of three trials.
Figure 3
Figure 3
(A) B. splendens erythrophore cell response after 6 h exposure to bacterial isolate. A negative change in pigment area is indicative of intracellular pigment aggregation whereas a positive change in pigment area represents pigment dispersion. (●) C. botulinum NCTC 7272; (◆) C. botulinum NCTC 7273; (▴) BHI medium. Data represent the mean values of three trials. (B) and (C) B. splendens erythrophore cells at 100×, the size bar represents a length of 100 µm; Left, time = 0 h, Right, time = 6 h, exposure to: (B) BHI medium, (C) C. botulinum NCTC 7272.
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