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Link to original content: http://pubmed.ncbi.nlm.nih.gov/18425128/
A structural-maintenance-of-chromosomes hinge domain-containing protein is required for RNA-directed DNA methylation - PubMed Skip to main page content
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. 2008 May;40(5):670-5.
doi: 10.1038/ng.119. Epub 2008 Apr 20.

A structural-maintenance-of-chromosomes hinge domain-containing protein is required for RNA-directed DNA methylation

Tatsuo Kanno  1 , Etienne Bucher, Lucia Daxinger, Bruno Huettel, Gudrun Böhmdorfer, Wolfgang Gregor, David P Kreil, Marjori Matzke, Antonius J M Matzke
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A structural-maintenance-of-chromosomes hinge domain-containing protein is required for RNA-directed DNA methylation

Tatsuo Kanno et al. Nat Genet. 2008 May.

Abstract

RNA-directed DNA methylation (RdDM) is a process in which dicer-generated small RNAs guide de novo cytosine methylation at the homologous DNA region. To identify components of the RdDM machinery important for Arabidopsis thaliana development, we targeted an enhancer active in meristems for methylation, which resulted in silencing of a downstream GFP reporter gene. This silencing system also features secondary siRNAs, which trigger methylation that spreads beyond the targeted enhancer region. A screen for mutants defective in meristem silencing and enhancer methylation retrieved six dms complementation groups, which included the known factors DRD1 (ref. 3; a SNF2-like chromatin-remodeling protein) and Pol IVb subunits. Additionally, we identified a previously unknown gene DMS3 (At3g49250), encoding a protein similar to the hinge-domain region of structural maintenance of chromosomes (SMC) proteins. This finding implicates a putative chromosome architectural protein that can potentially link nucleic acids in facilitating an RNAi-mediated epigenetic modification involving secondary siRNAs and spreading of DNA methylation.

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