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Link to original content: http://pubmed.ncbi.nlm.nih.gov/15623485/
Distribution of HPV infection and tumour markers in cervical intraepithelial neoplasia from cone biopsies of Mozambican women - PubMed Skip to main page content
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. 2005 Jan;58(1):61-8.
doi: 10.1136/jcp.2004.020552.

Distribution of HPV infection and tumour markers in cervical intraepithelial neoplasia from cone biopsies of Mozambican women

C Carrilho  1 , L Cirnes, M Alberto, L Buane, N Mendes, L David
Affiliations

Distribution of HPV infection and tumour markers in cervical intraepithelial neoplasia from cone biopsies of Mozambican women

C Carrilho et al. J Clin Pathol. 2005 Jan.

Abstract

Aims: To evaluate human papillomavirus (HPV) infection in whole cervical cone specimens with cervical intraepithelial neoplasia (CIN). In addition, to evaluate the relation between the presence of CIN lesions and HPV infection and the expression of Ki-67, p53, cytokeratins, Gp230 glycoprotein, and simple mucin-type carbohydrates.

Methods: Cervical cone specimens from five patients with CIN were studied. For each specimen, serial sections encompassing the whole cone were collected (52 samples). HPV infection and HPV types were detected by the polymerase chain reaction and enzyme immunoassay. The expression of Ki-67, p53, cytokeratins, Gp230, and simple mucin-type carbohydrates was examined immunohistochemically.

Results: All cases showed high risk HPV types, namely types 16, 33, 35, and 58. Four of the five patients were infected by multiple viral types. HPV-58 was always seen in CIN III, whereas HPV-35 was more frequent in CIN I. The expression of Ki-67 and p53 was higher in CIN III lesions. The expression of cytokeratins 8 and 17 showed complete or almost complete overlap with CIN III. Altered expression of Gp230, Tn, and sialyl-T was often seen in all grades of CIN.

Conclusions: When whole cervical cone specimens are evaluated the rate of multiple HPV infection is very high. The expression of cytokeratins 8 and 17 is a useful marker of CIN III.

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Figures

Figure 1
Figure 1
Schematic representation of case 1, including the absence or presence of human papillomavirus (HPV) and HPV types in the nine sections. Lesions in the whole specimen are outlined in the outer circle, with cervical intraepithelial neoplasia (CIN) III lesions marked with a thicker line. Different cytokeratins (Cks) and glycoprotein markers are outlined in the inner circles whenever their pattern of expression was abnormal.
Figure 2
Figure 2
Schematic representation of case 2, including the absence or presence of human papillomavirus (HPV) and HPV types in the 11 sections. Lesions in the whole specimen are outlined in the outer circle, with cervical intraepithelial neoplasia (CIN) II and III lesions marked with a thicker line. Different cytokeratins (Cks) and glycoprotein markers are outlined in the inner circles whenever their pattern of expression was abnormal.
Figure 3
Figure 3
Schematic representation of case 3, including the absence or presence of human papillomavirus (HPV) and HPV types in the 13 sections. Lesions of cervical intraepithelial neoplasia (CIN) I were observed in the whole specimen and are outlined in the outer circle. Different cytokeratins (Cks) and glycoprotein markers are outlined in the inner circles whenever their pattern of expression was abnormal.
Figure 4
Figure 4
Schematic representation of case 4, including the absence or presence of human papillomavirus (HPV) and HPV types in the seven sections. Lesions of cervical intraepithelial neoplasia (CIN) III were observed in the whole specimen and are outlined in the outer circle. Different cytokeratins (Cks) and glycoprotein markers are outlined in the inner circles whenever their pattern of expression was abnormal.
Figure 5
Figure 5
Schematic representation of case 5, including the absence or presence of human papillomavirus (HPV) and HPV types in the 12 sections. Lesions in the whole specimen are outlined in the outer circle, with cervical intraepithelial neoplasia (CIN) III lesions marked with a thicker line. Different cytokeratins (Cks) and glycoprotein markers are outlined in the inner circles whenever their pattern of expression was abnormal.
Figure 6
Figure 6
Immunohistochemical staining patterns in normal cervix and cervical lesions. (A) Ki-67 staining in many nuclei of a cervical intraepithelial neoplasia (CIN) III lesion; (B) CIN III lesion expressing cytokeratin 8 (Ck8); endocervical glands also express Ck8; (C) normal cervix expressing Ck13 in the suprabasal layers; (D) CIN III lesion with loss of expression of Ck13; (E) normal cervix expressing Gp230 in the suprabasal layers; (F) CIN III lesion with loss of expression of Gp230.
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