PROSITE documentation PDOC51723Peptidase family M60 domain profile
The peptidase family M60 domain belongs to the zincin superfamily of zinc-requiring metalloproteases (clan MA, subclan MA(E)) [E1]. The peptidase family M60 domain contains the metal-binding consensus motif HExxH. The two histidine residues are ligands of the catalytic Zn(2+) and the glutamic acid residue is involved in nucleophilic attack. An additional conserved glutamic acid is found approximately 20 residues from the first histidine within the metal-binding motif. The second conserved glutamic acid potentially acts as a third proteous Zn(2+) ligand. The peptidase family M60 domain targets complex host glycoproteins, such as mucins [1,2,3,4,5].
Some proteins known to contain a peptidase family M60 domain are listed below:
- Baculovirus viral enhancing factor (VEF), also known as Enhancin, a metalloprotease that enhances viral infection by degrading the peritrophic membrane (PM) of the insect midgut.
- Bacteria enhancin-like metalloproteases.
- Mammalian FAM115 family proteins.
The profile we developed covers the entire peptidase family M60 domain.
Last update:June 2014 / First entry.
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PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Bischoff D.S. Slavicek J.M. |
| Title | Molecular analysis of an enhancin gene in the Lymantria dispar nuclear polyhedrosis virus. | |
| Source | J. Virol. 71:8133-8140(1997). | |
| PubMed ID | 9343163 |
| 2 | Authors | Galloway C.S. Wang P. Winstanley D. Jones I.M. |
| Title | Comparison of the bacterial Enhancin-like proteins from Yersinia and Bacillus spp. with a baculovirus Enhancin. | |
| Source | J. Invertebr. Pathol. 90:134-137(2005). | |
| PubMed ID | 16081094 | |
| DOI | 10.1016/j.jip.2005.06.008 |
| 3 | Authors | Hajaij-Ellouze M. Fedhila S. Lereclus D. Nielsen-LeRoux C. |
| Title | The enhancin-like metalloprotease from the Bacillus cereus group is regulated by the pleiotropic transcriptional activator PlcR but is not essential for larvicidal activity. | |
| Source | FEMS Microbiol. Lett. 260:9-16(2006). | |
| PubMed ID | 16790012 | |
| DOI | 10.1111/j.1574-6968.2006.00289.x |
| 4 | Authors | Toprak U. Harris S. Baldwin D. Theilmann D. Gillott C. Hegedus D.D. Erlandson M.A. |
| Title | Role of enhancin in Mamestra configurata nucleopolyhedrovirus virulence: selective degradation of host peritrophic matrix proteins. | |
| Source | J. Gen. Virol. 93:744-753(2012). | |
| PubMed ID | 22238230 | |
| DOI | 10.1099/vir.0.038117-0 |
| 5 | Authors | Nakjang S. Ndeh D.A. Wipat A. Bolam D.N. Hirt R.P. |
| Title | A novel extracellular metallopeptidase domain shared by animal host-associated mutualistic and pathogenic microbes. | |
| Source | PLoS ONE 7:E30287-E30287(2012). | |
| PubMed ID | 22299034 | |
| DOI | 10.1371/journal.pone.0030287 |
| E1 | Title | https://www.ebi.ac.uk/merops/cgi-bin/famsum?family=M60 |
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