PROSITE documentation PDOC01027Glycosyl hydrolases family 4 signature
Description
It has been shown [1,2,E1] that the following glycosyl hydrolases can be, on the basis of sequence similarities, classified into a single family:
- Escherichia coli and Bacillus subtilis α-galactosidase (EC 3.2.1.22) (melibiase) (gene melA).
- Thermotoga maritima α-glucosidase (EC 3.2.1.20) (gene aglA).
- Probable 6-phospho-β-glucosidases (EC 3.2.1.86) (gene celF) from Escherichia coli and Bacillus subtilis.
- Maltose-6'-phosphate glucosidases (EC 3.2.1.122) (6-phospho-α-D- glucosidase) from Bacillus subtilis (gene glvG) and Fusobacterium mortiferum (gene malH).
- Bacillus subtilis protein lplD.
These enzymes require NAD and a divalent ion for their activity. They are proteins of about 50 Kd. As a signature pattern we selected a conserved region located in the central section. This region does not contain residues directly shown to be important for the catalytic activity.
Expert(s) to contact by email: Last update:April 2006 / Pattern revised.
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Technical section
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References
| 1 | Authors | Henrissat B. |
| Title | A classification of glycosyl hydrolases based on amino acid sequence similarities. | |
| Source | Biochem. J. 280:309-316(1991). | |
| PubMed ID | 1747104 |
| 2 | Authors | Thompson J. Pikis A. Ruvinov S.B. Henrissat B. Yamamoto H. Sekiguchi J. |
| Title | The gene glvA of Bacillus subtilis 168 encodes a metal-requiring, NAD(H)-dependent 6-phospho-alpha-glucosidase. Assignment to family 4 of the glycosylhydrolase superfamily. | |
| Source | J. Biol. Chem. 273:27347-27356(1998). | |
| PubMed ID | 9765262 |
| E1 | Title | https://www.uniprot.org/docs/glycosid |
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