PROSITE documentation PDOC00088N-4 cytosine-specific DNA methylases signature
N-4 cytosine-specific DNA methylases (EC 2.1.1.113) [1,2,3] are enzymes that specifically methylate the amino group at the C-4 position of cytosines in DNA. Such enzymes are found as components of type II restriction-modification systems in prokaryotes. Such enzymes recognize a specific sequence in DNA and methylate a cytosine in that sequence. By this action they protect DNA from cleavage by type II restriction enzymes that recognize the same sequence.
Type II N-4 Mtases seem to be structurally and enzymatically related to N-6 adenine-specific DNA methylases. Like the N-6 Mtases they contain a conserved Pro-Pro-Tyr/Phe region, but the N- and C-terminal contexts of this region are sufficiently different to derive a consensus pattern specific to this type of enzymes.
Expert(s) to contact by email: Last update:November 1997 / Text revised.
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| 1 | Authors | Tao T. Walter J. Brennan K.J. Cotterman M.M. Blumenthal R.M. |
| Title | Sequence, internal homology and high-level expression of the gene for a DNA-(cytosine N4)-methyltransferase, M.Pvu II. | |
| Source | Nucleic Acids Res. 17:4161-4175(1989). | |
| PubMed ID | 2662138 |
| 2 | Authors | Klimasauskas S. Timinskas A. Menkevicius S. Butkiene D. Butkus V. Janulaitis A. |
| Title | Sequence motifs characteristic of DNA[cytosine-N4]methyltransferases: similarity to adenine and cytosine-C5 DNA-methylases. | |
| Source | Nucleic Acids Res. 17:9823-9832(1989). | |
| PubMed ID | 2690010 |
| 3 | Authors | Timinskas A. Butkus V. Janulaitis A. |
| Title | Sequence motifs characteristic for DNA [cytosine-N4] and DNA [adenine-N6] methyltransferases. Classification of all DNA methyltransferases. | |
| Source | Gene 157:3-11(1995). | |
| PubMed ID | 7607512 |
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